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Phage Peptide Libraries As a Source of Targeted Ligands.

Nemudraya AA, Richter VA, Kuligina EV - Acta Naturae (2016 Jan-Mar)

Bottom Line: One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs.This problem is particularly acute in relation to oncologic diseases.Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia.

ABSTRACT
One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs. This problem is particularly acute in relation to oncologic diseases. Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer. The review discusses in detail the technology of phage display as a method for obtaining specific targeted peptide agents and offers examples of their use in diagnostic and clinical practice.

No MeSH data available.


Related in: MedlinePlus

The structure of a bacteriophage with a displayed foreign amino acid sequencein a pIII surface protein (indicated in red). N1, N2 and C – domains ofthe pIII surface protein
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Figure 1: The structure of a bacteriophage with a displayed foreign amino acid sequencein a pIII surface protein (indicated in red). N1, N2 and C – domains ofthe pIII surface protein

Mentions: Phage display technology, first proposed by G.P. Smith in 1985, played animportant role in the development of fundamentally new approaches in molecularbiology and opened up new opportunities for the development of thepharmaceutical industry. The concept of phage display lies in the cloning of aforeign DNA sequence into a specific site of a bacteriophage surface proteingene so that this sequence shares the reading frame with the protein. Theresult is a chimeric protein containing a foreign amino acid sequence formed(displayed) on the surface of the bacteriophage(Fig. 1).Furthermore, the physiological properties and viability of the viral particleare preserved [4, 5].


Phage Peptide Libraries As a Source of Targeted Ligands.

Nemudraya AA, Richter VA, Kuligina EV - Acta Naturae (2016 Jan-Mar)

The structure of a bacteriophage with a displayed foreign amino acid sequencein a pIII surface protein (indicated in red). N1, N2 and C – domains ofthe pIII surface protein
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837571&req=5

Figure 1: The structure of a bacteriophage with a displayed foreign amino acid sequencein a pIII surface protein (indicated in red). N1, N2 and C – domains ofthe pIII surface protein
Mentions: Phage display technology, first proposed by G.P. Smith in 1985, played animportant role in the development of fundamentally new approaches in molecularbiology and opened up new opportunities for the development of thepharmaceutical industry. The concept of phage display lies in the cloning of aforeign DNA sequence into a specific site of a bacteriophage surface proteingene so that this sequence shares the reading frame with the protein. Theresult is a chimeric protein containing a foreign amino acid sequence formed(displayed) on the surface of the bacteriophage(Fig. 1).Furthermore, the physiological properties and viability of the viral particleare preserved [4, 5].

Bottom Line: One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs.This problem is particularly acute in relation to oncologic diseases.Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia.

ABSTRACT
One of the dominant trends in modern pharmacology is the creation of drugs that act directly on the lesion focus and have minimal toxicity on healthy tissues and organs. This problem is particularly acute in relation to oncologic diseases. Short tissue- and organ-specific peptides capable of delivering drugs to the affected organ or tissue are considered promising targeted agents that can be used in the diagnosis and therapy of diseases, including cancer. The review discusses in detail the technology of phage display as a method for obtaining specific targeted peptide agents and offers examples of their use in diagnostic and clinical practice.

No MeSH data available.


Related in: MedlinePlus