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Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus

Structure of hnRNP particles. (right-hand panel) RNA- and protein-labeled30S particles (the latter labelled with 125I) before and after treatmentwith 2M NaCl. In contrast to the initial particles, those treated with 2M NaCllost all total RNA, although their sedimentation coefficient and EM dimensionsremained unchanged. Buoyant density decreased from 1.4 to 1.34 g/ml. (lefthandpanel) EM of dissociated and reconstructed 30S particles. (bottom panel)The scheme of dissociation and reconstruction of hnRNP.
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Figure 5: Structure of hnRNP particles. (right-hand panel) RNA- and protein-labeled30S particles (the latter labelled with 125I) before and after treatmentwith 2M NaCl. In contrast to the initial particles, those treated with 2M NaCllost all total RNA, although their sedimentation coefficient and EM dimensionsremained unchanged. Buoyant density decreased from 1.4 to 1.34 g/ml. (lefthandpanel) EM of dissociated and reconstructed 30S particles. (bottom panel)The scheme of dissociation and reconstruction of hnRNP.

Mentions: To better understand the structure of hnRNP particles, we elucidated thestructure of the 30S monomer. Intense ribonuclease treatment completelydestroyed the 30S particle RNA, which suggests that it is localized on theparticle’s surface. The 30S proteins were labeled with 125I,and the particles were treated with 2M NaCl to cause dissociation of RNA andthe protein. After ultracentrifugation, all of the hnRNA remained in the upperfractions, whereas the protein was detected in the same 30S peak, despite theremoval of RNA. The buoyant density of 30S particles dropped to 1.34 g/cm3[1-6](Fig. 5).


Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Structure of hnRNP particles. (right-hand panel) RNA- and protein-labeled30S particles (the latter labelled with 125I) before and after treatmentwith 2M NaCl. In contrast to the initial particles, those treated with 2M NaCllost all total RNA, although their sedimentation coefficient and EM dimensionsremained unchanged. Buoyant density decreased from 1.4 to 1.34 g/ml. (lefthandpanel) EM of dissociated and reconstructed 30S particles. (bottom panel)The scheme of dissociation and reconstruction of hnRNP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837567&req=5

Figure 5: Structure of hnRNP particles. (right-hand panel) RNA- and protein-labeled30S particles (the latter labelled with 125I) before and after treatmentwith 2M NaCl. In contrast to the initial particles, those treated with 2M NaCllost all total RNA, although their sedimentation coefficient and EM dimensionsremained unchanged. Buoyant density decreased from 1.4 to 1.34 g/ml. (lefthandpanel) EM of dissociated and reconstructed 30S particles. (bottom panel)The scheme of dissociation and reconstruction of hnRNP.
Mentions: To better understand the structure of hnRNP particles, we elucidated thestructure of the 30S monomer. Intense ribonuclease treatment completelydestroyed the 30S particle RNA, which suggests that it is localized on theparticle’s surface. The 30S proteins were labeled with 125I,and the particles were treated with 2M NaCl to cause dissociation of RNA andthe protein. After ultracentrifugation, all of the hnRNA remained in the upperfractions, whereas the protein was detected in the same 30S peak, despite theremoval of RNA. The buoyant density of 30S particles dropped to 1.34 g/cm3[1-6](Fig. 5).

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus