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Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus

Properties of nuclear hnRNP polyparticles. (left panel) Distribution ofhnRNP particles isolated with a RNAase inhibitor at ultracentrifugation. Conversionof polyparticles into 30S monomers with mild RNAase treatment. (toppanel) Polyparticles, molecular weights of RNA extracted from particles withdifferent numbers of monomers, and the number of nucleotide monomers perparticle. (bottom right panel) EM of the particles from different areas of thegradient, sedimentation coefficients, and number of monomers. The buoyantdensity is the same for all particles (1.4)
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Figure 4: Properties of nuclear hnRNP polyparticles. (left panel) Distribution ofhnRNP particles isolated with a RNAase inhibitor at ultracentrifugation. Conversionof polyparticles into 30S monomers with mild RNAase treatment. (toppanel) Polyparticles, molecular weights of RNA extracted from particles withdifferent numbers of monomers, and the number of nucleotide monomers perparticle. (bottom right panel) EM of the particles from different areas of thegradient, sedimentation coefficients, and number of monomers. The buoyantdensity is the same for all particles (1.4)

Mentions: After ultracentrifugation, most of the hnRNA was detected in a homogeneous 30Speak, which contained particles ca. 20 nm in diameter. The molecular weight ofRNA isolated from the 30S peak was low(Fig. 3). It wascontrary to the data on the very high molecular mass of hnRNK isolated byphenol fractionation. To resolve this contradiction, we performed extraction inthe presence of a RNAase inhibitor from rat liver supernatant. This extractionproduced a completely different pattern of ultracentrifugation: a series ofpeaks ranging from a small 30S peak all the way up to material withsedimentation coefficients of 200S and above(Fig. 4).Obviously, this pattern was much closer to the native one[14].


Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Properties of nuclear hnRNP polyparticles. (left panel) Distribution ofhnRNP particles isolated with a RNAase inhibitor at ultracentrifugation. Conversionof polyparticles into 30S monomers with mild RNAase treatment. (toppanel) Polyparticles, molecular weights of RNA extracted from particles withdifferent numbers of monomers, and the number of nucleotide monomers perparticle. (bottom right panel) EM of the particles from different areas of thegradient, sedimentation coefficients, and number of monomers. The buoyantdensity is the same for all particles (1.4)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837567&req=5

Figure 4: Properties of nuclear hnRNP polyparticles. (left panel) Distribution ofhnRNP particles isolated with a RNAase inhibitor at ultracentrifugation. Conversionof polyparticles into 30S monomers with mild RNAase treatment. (toppanel) Polyparticles, molecular weights of RNA extracted from particles withdifferent numbers of monomers, and the number of nucleotide monomers perparticle. (bottom right panel) EM of the particles from different areas of thegradient, sedimentation coefficients, and number of monomers. The buoyantdensity is the same for all particles (1.4)
Mentions: After ultracentrifugation, most of the hnRNA was detected in a homogeneous 30Speak, which contained particles ca. 20 nm in diameter. The molecular weight ofRNA isolated from the 30S peak was low(Fig. 3). It wascontrary to the data on the very high molecular mass of hnRNK isolated byphenol fractionation. To resolve this contradiction, we performed extraction inthe presence of a RNAase inhibitor from rat liver supernatant. This extractionproduced a completely different pattern of ultracentrifugation: a series ofpeaks ranging from a small 30S peak all the way up to material withsedimentation coefficients of 200S and above(Fig. 4).Obviously, this pattern was much closer to the native one[14].

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus