Limits...
Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus

Characteristics of nuclear dRNA. (left-hand panel) Ultracentrifugation ofnuclear dRNA labeled for 1 h with 32P in a sucrose density gradient. Significantlyhigher molecular weight of the labeled dRNA than that of total dRNA as determinedby optical density. Here and later, thin arrows indicate the directionof ultracentrifugation. (central panel) Comparison of the molecular weights ofnuclear dRNA and cytoplasmic mRNA labelled in identical conditions (1 h). Theformer has a much higher molecular weight. (right-hand panel) Hybridization oflabeled cytoplasmic mRNA and nuclear dRNA with DNA and competition withunlabeled nuclear dRNA and cytoplasmic mRNA
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4837567&req=5

Figure 2: Characteristics of nuclear dRNA. (left-hand panel) Ultracentrifugation ofnuclear dRNA labeled for 1 h with 32P in a sucrose density gradient. Significantlyhigher molecular weight of the labeled dRNA than that of total dRNA as determinedby optical density. Here and later, thin arrows indicate the directionof ultracentrifugation. (central panel) Comparison of the molecular weights ofnuclear dRNA and cytoplasmic mRNA labelled in identical conditions (1 h). Theformer has a much higher molecular weight. (right-hand panel) Hybridization oflabeled cytoplasmic mRNA and nuclear dRNA with DNA and competition withunlabeled nuclear dRNA and cytoplasmic mRNA

Mentions: Next, we described the properties of nuclear dRNA. Its molecular mass washighly heterogeneous and reached very high values. The nascent nuclear dRNA hada significantly higher molecular mass than the total nuclear dRNA, whichimplied its cleavage in the cell nucleus (processing)[7, 8](Fig. 2).


Discovery of Nuclear DNA-like RNA (dRNA, hnRNA) and Ribonucleoproteins Particles Containing hnRNA.

Georgiev GP - Acta Naturae (2016 Jan-Mar)

Characteristics of nuclear dRNA. (left-hand panel) Ultracentrifugation ofnuclear dRNA labeled for 1 h with 32P in a sucrose density gradient. Significantlyhigher molecular weight of the labeled dRNA than that of total dRNA as determinedby optical density. Here and later, thin arrows indicate the directionof ultracentrifugation. (central panel) Comparison of the molecular weights ofnuclear dRNA and cytoplasmic mRNA labelled in identical conditions (1 h). Theformer has a much higher molecular weight. (right-hand panel) Hybridization oflabeled cytoplasmic mRNA and nuclear dRNA with DNA and competition withunlabeled nuclear dRNA and cytoplasmic mRNA
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837567&req=5

Figure 2: Characteristics of nuclear dRNA. (left-hand panel) Ultracentrifugation ofnuclear dRNA labeled for 1 h with 32P in a sucrose density gradient. Significantlyhigher molecular weight of the labeled dRNA than that of total dRNA as determinedby optical density. Here and later, thin arrows indicate the directionof ultracentrifugation. (central panel) Comparison of the molecular weights ofnuclear dRNA and cytoplasmic mRNA labelled in identical conditions (1 h). Theformer has a much higher molecular weight. (right-hand panel) Hybridization oflabeled cytoplasmic mRNA and nuclear dRNA with DNA and competition withunlabeled nuclear dRNA and cytoplasmic mRNA
Mentions: Next, we described the properties of nuclear dRNA. Its molecular mass washighly heterogeneous and reached very high values. The nascent nuclear dRNA hada significantly higher molecular mass than the total nuclear dRNA, whichimplied its cleavage in the cell nucleus (processing)[7, 8](Fig. 2).

Bottom Line: Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics.However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N.The transcript of the lecture is presented below.

View Article: PubMed Central - PubMed

Affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

ABSTRACT
On August 9-11, 2014, Cold Spring Harbor (USA) hosted a special symposium dedicated to the discovery of messenger or informational RNA and the main events in the subsequent studies of its synthesis, regulation of synthesis, maturation, and transport. The existence of mRNA in bacteria was first suggested in 1961 by Jacob and Monod, based on genetic studies [1]. The same year, Brenner et al. confirmed the hypothesis [2]. Our laboratory played a key role in the discovery of messenger RNA in eukaryotes, as well as in the discovery of the nuclear ribonucleoproteins that contain it and in the elucidation of their structural organization. Therefore, I was invited to represent Russia at the Symposium and deliver a speech on these topics. However, my visa had only been issued after the end of the Symposium, and, therefore, the presentation was delivered by my former colleague G.N. Yenikolopov, who works at Cold Spring Harbor Laboratory. The transcript of the lecture is presented below.

No MeSH data available.


Related in: MedlinePlus