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Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus

Aged female mice supplemented with L. reuteri strain BM36301 display healthy skins. a Hair re-growth experiment on C57BL/6 mice consuming L. reuteri strain-treated water or control water. A 2 × 2 cm wide area of skin was clearly shaved and the hair re-growth was examined a week later. b Hair follicle (HF) counts were collected from the skin section samples of mice fed with either control or L. reuteri strains. Five microscopic images at 400× resolution from 5 to 8 mice were counted. * indicates p = 0.023 from the t-test in comparison with the control group (female lanes 1 and 2). c Skin sections from control (left) and BM36301-treated (right) mice were stained with Hematoxylin and Eosin to show skin tissue layers and HFs as labeled. Photos were taken at 100× resolution; the bar indicates of 100 μm in length for scale
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Fig5: Aged female mice supplemented with L. reuteri strain BM36301 display healthy skins. a Hair re-growth experiment on C57BL/6 mice consuming L. reuteri strain-treated water or control water. A 2 × 2 cm wide area of skin was clearly shaved and the hair re-growth was examined a week later. b Hair follicle (HF) counts were collected from the skin section samples of mice fed with either control or L. reuteri strains. Five microscopic images at 400× resolution from 5 to 8 mice were counted. * indicates p = 0.023 from the t-test in comparison with the control group (female lanes 1 and 2). c Skin sections from control (left) and BM36301-treated (right) mice were stained with Hematoxylin and Eosin to show skin tissue layers and HFs as labeled. Photos were taken at 100× resolution; the bar indicates of 100 μm in length for scale

Mentions: One interesting feature of certain probiotics is that they can promote skin health in a manner dependent on immune regulation [21, 38]. Since the BM36301 also showed anti-inflammatory effects in vitro and in vivo, we asked the question whether this strain can provoke such health benefits in skin. At the 18th week of treatment, we shaved an area on 2 mice from each group and examined them after 1 week. Interestingly, BM36301-fed female mice exhibited faster hair re-growth, though not full recovery, at the shaved area (Fig. 5a). In contrast, control or BM36304 groups did not display such a rapid pace of recovery. None of the treated males showed hair re-growth distinguishably faster than the control, indicating that this accelerated hair growth may be obvious in only females. Another metric to evaluate skin health is by examining fur shininess. However, we did not observe meaningful differences between each group through sensory or light meter measurements, mainly due to the relatively shiny fur conditions of the controls (data not shown).Fig. 5


Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

Aged female mice supplemented with L. reuteri strain BM36301 display healthy skins. a Hair re-growth experiment on C57BL/6 mice consuming L. reuteri strain-treated water or control water. A 2 × 2 cm wide area of skin was clearly shaved and the hair re-growth was examined a week later. b Hair follicle (HF) counts were collected from the skin section samples of mice fed with either control or L. reuteri strains. Five microscopic images at 400× resolution from 5 to 8 mice were counted. * indicates p = 0.023 from the t-test in comparison with the control group (female lanes 1 and 2). c Skin sections from control (left) and BM36301-treated (right) mice were stained with Hematoxylin and Eosin to show skin tissue layers and HFs as labeled. Photos were taken at 100× resolution; the bar indicates of 100 μm in length for scale
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837529&req=5

Fig5: Aged female mice supplemented with L. reuteri strain BM36301 display healthy skins. a Hair re-growth experiment on C57BL/6 mice consuming L. reuteri strain-treated water or control water. A 2 × 2 cm wide area of skin was clearly shaved and the hair re-growth was examined a week later. b Hair follicle (HF) counts were collected from the skin section samples of mice fed with either control or L. reuteri strains. Five microscopic images at 400× resolution from 5 to 8 mice were counted. * indicates p = 0.023 from the t-test in comparison with the control group (female lanes 1 and 2). c Skin sections from control (left) and BM36301-treated (right) mice were stained with Hematoxylin and Eosin to show skin tissue layers and HFs as labeled. Photos were taken at 100× resolution; the bar indicates of 100 μm in length for scale
Mentions: One interesting feature of certain probiotics is that they can promote skin health in a manner dependent on immune regulation [21, 38]. Since the BM36301 also showed anti-inflammatory effects in vitro and in vivo, we asked the question whether this strain can provoke such health benefits in skin. At the 18th week of treatment, we shaved an area on 2 mice from each group and examined them after 1 week. Interestingly, BM36301-fed female mice exhibited faster hair re-growth, though not full recovery, at the shaved area (Fig. 5a). In contrast, control or BM36304 groups did not display such a rapid pace of recovery. None of the treated males showed hair re-growth distinguishably faster than the control, indicating that this accelerated hair growth may be obvious in only females. Another metric to evaluate skin health is by examining fur shininess. However, we did not observe meaningful differences between each group through sensory or light meter measurements, mainly due to the relatively shiny fur conditions of the controls (data not shown).Fig. 5

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus