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Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus

Aged male mice supplemented with L. reuteri strain BM36301 maintain healthy body index. a Net weight gain of mice C57BL/6 (n = 6 for male, grey bar; n = 8 for female, dark bar) fed with each L. reuteri strain for 20 week period. * indicates p = 0.040 from the t-test in comparison with the control group (male lanes 1 and 2). b Serum insulin concentration from mice fed with each L. reuteri strain as in (a). ** indicates p = 0.027 from the t-test in comparison with the control group (male lanes 1 and 3). c Serum testosterone amount from male mice fed with each L. reuteri strain as in (a). *** indicates p = 0.0025 from the t-test in comparison with the control group (lanes 1 and 2)
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Fig4: Aged male mice supplemented with L. reuteri strain BM36301 maintain healthy body index. a Net weight gain of mice C57BL/6 (n = 6 for male, grey bar; n = 8 for female, dark bar) fed with each L. reuteri strain for 20 week period. * indicates p = 0.040 from the t-test in comparison with the control group (male lanes 1 and 2). b Serum insulin concentration from mice fed with each L. reuteri strain as in (a). ** indicates p = 0.027 from the t-test in comparison with the control group (male lanes 1 and 3). c Serum testosterone amount from male mice fed with each L. reuteri strain as in (a). *** indicates p = 0.0025 from the t-test in comparison with the control group (lanes 1 and 2)

Mentions: Aging is the source of many health issues in humans, including diabetes and obesity. In order to exacerbate such aging issues in the animal model, high fat diets are often employed [35, 36]. However, the interpretation on those accelerated experiments can be complicated since no aging in humans should be driven by a purposed high fat diet. We cultivated all the mice with a standard diet so that their aging process could be most natural. In the 20-week experiment timespan starting from the age of 20 weeks old, control group males gained about 8 g weight (7.83 ± 1.2 g) and control group females gained about 5 g weight (4.8 ± 0.94 g). Remarkably, it was noticeable that the weight gain in males fed with the anti-inflammatory BM36301 was significantly lower than in the control (5.78 ± 0.75 g, p = 0.040) by 36 % (Fig. 4a, lanes 1 and 2). However, the weight gain of BM36304-treated males (7.53 ± 0.74 g) was not significantly different from the control (p = 0.67). The weight gains of the female groups were not statistically different from each other.Fig. 4


Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

Aged male mice supplemented with L. reuteri strain BM36301 maintain healthy body index. a Net weight gain of mice C57BL/6 (n = 6 for male, grey bar; n = 8 for female, dark bar) fed with each L. reuteri strain for 20 week period. * indicates p = 0.040 from the t-test in comparison with the control group (male lanes 1 and 2). b Serum insulin concentration from mice fed with each L. reuteri strain as in (a). ** indicates p = 0.027 from the t-test in comparison with the control group (male lanes 1 and 3). c Serum testosterone amount from male mice fed with each L. reuteri strain as in (a). *** indicates p = 0.0025 from the t-test in comparison with the control group (lanes 1 and 2)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837529&req=5

Fig4: Aged male mice supplemented with L. reuteri strain BM36301 maintain healthy body index. a Net weight gain of mice C57BL/6 (n = 6 for male, grey bar; n = 8 for female, dark bar) fed with each L. reuteri strain for 20 week period. * indicates p = 0.040 from the t-test in comparison with the control group (male lanes 1 and 2). b Serum insulin concentration from mice fed with each L. reuteri strain as in (a). ** indicates p = 0.027 from the t-test in comparison with the control group (male lanes 1 and 3). c Serum testosterone amount from male mice fed with each L. reuteri strain as in (a). *** indicates p = 0.0025 from the t-test in comparison with the control group (lanes 1 and 2)
Mentions: Aging is the source of many health issues in humans, including diabetes and obesity. In order to exacerbate such aging issues in the animal model, high fat diets are often employed [35, 36]. However, the interpretation on those accelerated experiments can be complicated since no aging in humans should be driven by a purposed high fat diet. We cultivated all the mice with a standard diet so that their aging process could be most natural. In the 20-week experiment timespan starting from the age of 20 weeks old, control group males gained about 8 g weight (7.83 ± 1.2 g) and control group females gained about 5 g weight (4.8 ± 0.94 g). Remarkably, it was noticeable that the weight gain in males fed with the anti-inflammatory BM36301 was significantly lower than in the control (5.78 ± 0.75 g, p = 0.040) by 36 % (Fig. 4a, lanes 1 and 2). However, the weight gain of BM36304-treated males (7.53 ± 0.74 g) was not significantly different from the control (p = 0.67). The weight gains of the female groups were not statistically different from each other.Fig. 4

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus