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Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus

TNF-α from mice C57BL/6 supplemented with probiotic bacteria. a The TNF-α of the serum from male mice (n = 6 each) each fed with L. reuteri strain. ** indicates p = 0.006 from the t-test in comparison with the control group (lanes 1 and 3). b The TNF-α of the serum from female mice (n = 8 each) each fed with L. reuteri strain. * indicates p = 0.017 from the t-test in comparison with the control group (lanes 1 and 2)
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Fig3: TNF-α from mice C57BL/6 supplemented with probiotic bacteria. a The TNF-α of the serum from male mice (n = 6 each) each fed with L. reuteri strain. ** indicates p = 0.006 from the t-test in comparison with the control group (lanes 1 and 3). b The TNF-α of the serum from female mice (n = 8 each) each fed with L. reuteri strain. * indicates p = 0.017 from the t-test in comparison with the control group (lanes 1 and 2)

Mentions: We were primarily interested in the serum TNF-α levels from these mice. As shown in Fig. 3a, control males showed 5.85 ± 1.34 pg/ml of serum TNF-α. BM36301-treated males showed a slight reduction (4.90 ± 0.9 pg/ml), though without meaningful significance (p = 0.59). However, BM36304-treated males maintained significantly higher TNF-α levels (8.28 ± 1.09 pg/ml, p = 0.006 vs. control). We also quantitated the female’s TNF-α concentrations (Fig. 3b). We found that the female control group retained higher TNF-α levels (7.9 ± 1.05 pg/ml) than the male control group, though without significance (p = 0.42). Most notably, BM36301-fed females displayed significantly reduced amounts of TNF-α (5.17 ± 0.80 pg/ml, p = 0.017 vs. control), while BM36304-treated females showed similar levels with the control mice (7.43 ± 2.69 pg/ml, p = 0.71 vs. control). Unlike these variations of TNF-α, we could not discern meaningful differences of serum IL-10 levels between the groups (data not shown).Fig. 3


Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged mice.

Lee J, Yang W, Hostetler A, Schultz N, Suckow MA, Stewart KL, Kim DD, Kim HS - BMC Microbiol. (2016)

TNF-α from mice C57BL/6 supplemented with probiotic bacteria. a The TNF-α of the serum from male mice (n = 6 each) each fed with L. reuteri strain. ** indicates p = 0.006 from the t-test in comparison with the control group (lanes 1 and 3). b The TNF-α of the serum from female mice (n = 8 each) each fed with L. reuteri strain. * indicates p = 0.017 from the t-test in comparison with the control group (lanes 1 and 2)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4837529&req=5

Fig3: TNF-α from mice C57BL/6 supplemented with probiotic bacteria. a The TNF-α of the serum from male mice (n = 6 each) each fed with L. reuteri strain. ** indicates p = 0.006 from the t-test in comparison with the control group (lanes 1 and 3). b The TNF-α of the serum from female mice (n = 8 each) each fed with L. reuteri strain. * indicates p = 0.017 from the t-test in comparison with the control group (lanes 1 and 2)
Mentions: We were primarily interested in the serum TNF-α levels from these mice. As shown in Fig. 3a, control males showed 5.85 ± 1.34 pg/ml of serum TNF-α. BM36301-treated males showed a slight reduction (4.90 ± 0.9 pg/ml), though without meaningful significance (p = 0.59). However, BM36304-treated males maintained significantly higher TNF-α levels (8.28 ± 1.09 pg/ml, p = 0.006 vs. control). We also quantitated the female’s TNF-α concentrations (Fig. 3b). We found that the female control group retained higher TNF-α levels (7.9 ± 1.05 pg/ml) than the male control group, though without significance (p = 0.42). Most notably, BM36301-fed females displayed significantly reduced amounts of TNF-α (5.17 ± 0.80 pg/ml, p = 0.017 vs. control), while BM36304-treated females showed similar levels with the control mice (7.43 ± 2.69 pg/ml, p = 0.71 vs. control). Unlike these variations of TNF-α, we could not discern meaningful differences of serum IL-10 levels between the groups (data not shown).Fig. 3

Bottom Line: On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α.Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.It helped mice maintain healthy conditions as they aged.

View Article: PubMed Central - PubMed

Affiliation: Research and Development, Benebios LLC, 10527 Garden Grove Blvd, Garden Grove, CA, 92843, USA. joon.lee@benebios.com.

ABSTRACT

Background: The gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

Results: We have screened our collection of probiotic lactic acid bacteria (LAB) for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB's anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL/6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

Conclusion: The L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

No MeSH data available.


Related in: MedlinePlus