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UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens.

Zheng D, Wang Y, Han Y, Xu JR, Wang C - Sci Rep (2016)

Bottom Line: Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7.In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol.In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Rice false smut caused by Ustilaginoidea virens is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in U. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinase gene in U. virens. One Uvhog1 deletion mutant was identified after screening over 600 hygromycin-resistant transformants generated by Agrobacterium tumefaciens mediated transformation. The Uvhog1 mutant was reduced in growth rate and conidiation but had increased sensitivities to SDS, Congo red, and hyperosmotic stress. Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7. In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol. In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays. Overall, as the first report of targeted gene deletion mutant in U. virens, our results showed that UvHOG1 likely has conserved roles in regulating stress responses, hyphal growth, and possibly secondary metabolism.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of UvATF1, UvAP1, and UvSKN7.RNA samples were isolated from vegetative hyphae of UV-8b and the Uvhog1 mutant cultured in regular YT (CK) or YT with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. The expression level of (A) UvATF1, (B) UvSKN7, and (C)UvAP1 in the wild type strain cultured in regular YT was arbitrarily set to 1. Mean and standard deviations were calculated with results from three independent replicates.
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f3: Expression profiles of UvATF1, UvAP1, and UvSKN7.RNA samples were isolated from vegetative hyphae of UV-8b and the Uvhog1 mutant cultured in regular YT (CK) or YT with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. The expression level of (A) UvATF1, (B) UvSKN7, and (C)UvAP1 in the wild type strain cultured in regular YT was arbitrarily set to 1. Mean and standard deviations were calculated with results from three independent replicates.

Mentions: The ATF1, SKN7, and AP1 genes encode three transcription factors known to be involved in the regulation of stress-related genes in F. graminearum and other fungi303132. To determine whether deletion of UvHOG1 affects the expression of their orthologs in U. virens, RNA samples were isolated from hyphae of the wild-type strain UV-8b and Uvhog1 mutant that were harvested from 2-day-old regular YT cultures and cultures treated with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. In the wild type, the expression of UvAP1, UvATF1, and UvSKN7 had no significant change when treated with NaCl. However, SDS treatment resulted in a 50% increase in the expression of UvAP1 and UvSKN7, and H2O2 treatment caused an up-regulation of UvSKN7 expression (Fig. 3). In the Uvhog1 mutant, the expressions of all these three genes were reduced over two-folds by treatment with 0.5 M NaCl. Nevertheless, the expression of UvAP1 and UvSKN7 was up-regulated over two-folds in the presence of SDS (Fig. 3).


UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens.

Zheng D, Wang Y, Han Y, Xu JR, Wang C - Sci Rep (2016)

Expression profiles of UvATF1, UvAP1, and UvSKN7.RNA samples were isolated from vegetative hyphae of UV-8b and the Uvhog1 mutant cultured in regular YT (CK) or YT with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. The expression level of (A) UvATF1, (B) UvSKN7, and (C)UvAP1 in the wild type strain cultured in regular YT was arbitrarily set to 1. Mean and standard deviations were calculated with results from three independent replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837404&req=5

f3: Expression profiles of UvATF1, UvAP1, and UvSKN7.RNA samples were isolated from vegetative hyphae of UV-8b and the Uvhog1 mutant cultured in regular YT (CK) or YT with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. The expression level of (A) UvATF1, (B) UvSKN7, and (C)UvAP1 in the wild type strain cultured in regular YT was arbitrarily set to 1. Mean and standard deviations were calculated with results from three independent replicates.
Mentions: The ATF1, SKN7, and AP1 genes encode three transcription factors known to be involved in the regulation of stress-related genes in F. graminearum and other fungi303132. To determine whether deletion of UvHOG1 affects the expression of their orthologs in U. virens, RNA samples were isolated from hyphae of the wild-type strain UV-8b and Uvhog1 mutant that were harvested from 2-day-old regular YT cultures and cultures treated with 0.5 M NaCl, 0.03% SDS, or 0.07% H2O2. In the wild type, the expression of UvAP1, UvATF1, and UvSKN7 had no significant change when treated with NaCl. However, SDS treatment resulted in a 50% increase in the expression of UvAP1 and UvSKN7, and H2O2 treatment caused an up-regulation of UvSKN7 expression (Fig. 3). In the Uvhog1 mutant, the expressions of all these three genes were reduced over two-folds by treatment with 0.5 M NaCl. Nevertheless, the expression of UvAP1 and UvSKN7 was up-regulated over two-folds in the presence of SDS (Fig. 3).

Bottom Line: Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7.In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol.In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.

ABSTRACT
Rice false smut caused by Ustilaginoidea virens is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in U. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinase gene in U. virens. One Uvhog1 deletion mutant was identified after screening over 600 hygromycin-resistant transformants generated by Agrobacterium tumefaciens mediated transformation. The Uvhog1 mutant was reduced in growth rate and conidiation but had increased sensitivities to SDS, Congo red, and hyperosmotic stress. Deletion of UvHOG1 resulted in reduced expression of the stress response-related genes UvATF1 and UvSKN7. In the Uvhog1 mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol. In addition, the Uvhog1 mutant had reduced toxicity on shoot growth in rice seed germination assays. Overall, as the first report of targeted gene deletion mutant in U. virens, our results showed that UvHOG1 likely has conserved roles in regulating stress responses, hyphal growth, and possibly secondary metabolism.

No MeSH data available.


Related in: MedlinePlus