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Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts.

Liu YH, Kang ZW, Guo Y, Zhu GS, Shah MM, Song Y, Fan YL, Jing X, Liu TX - Sci Rep (2016)

Bottom Line: Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet.In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach.We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, and Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100 China.

ABSTRACT
Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet. Endosymbionts with EAAs synthesis functions are considered crucial for ameliorating the lack of EAAs in insects' diets. In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. The performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation were recorded. We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.

No MeSH data available.


Related in: MedlinePlus

Concentrations of individual amino acid and total free amino acids, the proportion of essential amino acids and amino acid composition profiles in the four plant phloem.(a) Concentrations of individual free amino acid (μM). (b) Amino acid composition profiles (PCA). (c) Concentration of total free amino acids (μM). (d) Proportion of essential amino acids (mol%). Asterisk or different letters above the error bars indicate significant differences among different treatments (P < 0.05, Tukey’s HSD test).
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f2: Concentrations of individual amino acid and total free amino acids, the proportion of essential amino acids and amino acid composition profiles in the four plant phloem.(a) Concentrations of individual free amino acid (μM). (b) Amino acid composition profiles (PCA). (c) Concentration of total free amino acids (μM). (d) Proportion of essential amino acids (mol%). Asterisk or different letters above the error bars indicate significant differences among different treatments (P < 0.05, Tukey’s HSD test).

Mentions: All protein amino acids were detected in phloem exudate samples of cabbage, eggplant, tobacco and spinach leaves (Fig. 2a). Cysteine was detected but not included in the analysis because the standard cannot dissolve in water.


Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts.

Liu YH, Kang ZW, Guo Y, Zhu GS, Shah MM, Song Y, Fan YL, Jing X, Liu TX - Sci Rep (2016)

Concentrations of individual amino acid and total free amino acids, the proportion of essential amino acids and amino acid composition profiles in the four plant phloem.(a) Concentrations of individual free amino acid (μM). (b) Amino acid composition profiles (PCA). (c) Concentration of total free amino acids (μM). (d) Proportion of essential amino acids (mol%). Asterisk or different letters above the error bars indicate significant differences among different treatments (P < 0.05, Tukey’s HSD test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837378&req=5

f2: Concentrations of individual amino acid and total free amino acids, the proportion of essential amino acids and amino acid composition profiles in the four plant phloem.(a) Concentrations of individual free amino acid (μM). (b) Amino acid composition profiles (PCA). (c) Concentration of total free amino acids (μM). (d) Proportion of essential amino acids (mol%). Asterisk or different letters above the error bars indicate significant differences among different treatments (P < 0.05, Tukey’s HSD test).
Mentions: All protein amino acids were detected in phloem exudate samples of cabbage, eggplant, tobacco and spinach leaves (Fig. 2a). Cysteine was detected but not included in the analysis because the standard cannot dissolve in water.

Bottom Line: Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet.In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach.We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, and Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100 China.

ABSTRACT
Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet. Endosymbionts with EAAs synthesis functions are considered crucial for ameliorating the lack of EAAs in insects' diets. In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. The performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation were recorded. We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.

No MeSH data available.


Related in: MedlinePlus