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Improved Anatomical Specificity of Non-invasive Neuro-stimulation by High Frequency (5 MHz) Ultrasound.

Li GF, Zhao HX, Zhou H, Yan F, Wang JY, Xu CX, Wang CZ, Niu LL, Meng L, Wu S, Zhang HL, Qiu WB, Zheng HR - Sci Rep (2016)

Bottom Line: Electromyography (EMG) collected from tail muscles together with the motion response videos were analyzed for evaluating the stimulation effects.Our results indicate that 5 MHz ultrasound can successfully achieve neuro-stimulation.It provides a smaller stimulation region, which offers improved anatomical specificity for neuro-stimulation in a non-invasive manner.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

ABSTRACT
Low frequency ultrasound (<1 MHz) has been demonstrated to be a promising approach for non-invasive neuro-stimulation. However, the focal width is limited to be half centimeter scale. Minimizing the stimulation region with higher frequency ultrasound will provide a great opportunity to expand its application. This study first time examines the feasibility of using high frequency (5 MHz) ultrasound to achieve neuro-stimulation in brain, and verifies the anatomical specificity of neuro-stimulation in vivo. 1 MHz and 5 MHz ultrasound stimulation were evaluated in the same group of mice. Electromyography (EMG) collected from tail muscles together with the motion response videos were analyzed for evaluating the stimulation effects. Our results indicate that 5 MHz ultrasound can successfully achieve neuro-stimulation. The equivalent diameter (ED) of the stimulation region with 5 MHz ultrasound (0.29 ± 0.08 mm) is significantly smaller than that with 1 MHz (0.83 ± 0.11 mm). The response latency of 5 MHz ultrasound (45 ± 31 ms) is also shorter than that of 1 MHz ultrasound (208 ± 111 ms). Consequently, high frequency (5 MHz) ultrasound can successfully activate the brain circuits in mice. It provides a smaller stimulation region, which offers improved anatomical specificity for neuro-stimulation in a non-invasive manner.

No MeSH data available.


HE stain for brain slices.(a) HE stain for control mouse without performing ultrasound stimulation. (b) HE stain for mouse under brain stimulation by 1 MHz ultrasound for 30 min. (c) HE stain for mouse under brain stimulation by 5 MHz ultrasound for 30 min. The ellipses indicate the regions where ultrasound stimulation occurred.
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f6: HE stain for brain slices.(a) HE stain for control mouse without performing ultrasound stimulation. (b) HE stain for mouse under brain stimulation by 1 MHz ultrasound for 30 min. (c) HE stain for mouse under brain stimulation by 5 MHz ultrasound for 30 min. The ellipses indicate the regions where ultrasound stimulation occurred.

Mentions: Figure 6a–c show the results of HE stain. The elliptical circles represent the ultrasound stimulation region. 1 MHz and 5 MHz ultrasound did not induce obvious morphological changes in the stimuli regions, as compared with the control mice. In addition, there is no obvious tissue bleeding or cell necrosis in the tissue stimulated by ultrasound. These results demonstrate that 1 MHz and 5 MHz ultrasound are safe for the neuro-stimulation.


Improved Anatomical Specificity of Non-invasive Neuro-stimulation by High Frequency (5 MHz) Ultrasound.

Li GF, Zhao HX, Zhou H, Yan F, Wang JY, Xu CX, Wang CZ, Niu LL, Meng L, Wu S, Zhang HL, Qiu WB, Zheng HR - Sci Rep (2016)

HE stain for brain slices.(a) HE stain for control mouse without performing ultrasound stimulation. (b) HE stain for mouse under brain stimulation by 1 MHz ultrasound for 30 min. (c) HE stain for mouse under brain stimulation by 5 MHz ultrasound for 30 min. The ellipses indicate the regions where ultrasound stimulation occurred.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837374&req=5

f6: HE stain for brain slices.(a) HE stain for control mouse without performing ultrasound stimulation. (b) HE stain for mouse under brain stimulation by 1 MHz ultrasound for 30 min. (c) HE stain for mouse under brain stimulation by 5 MHz ultrasound for 30 min. The ellipses indicate the regions where ultrasound stimulation occurred.
Mentions: Figure 6a–c show the results of HE stain. The elliptical circles represent the ultrasound stimulation region. 1 MHz and 5 MHz ultrasound did not induce obvious morphological changes in the stimuli regions, as compared with the control mice. In addition, there is no obvious tissue bleeding or cell necrosis in the tissue stimulated by ultrasound. These results demonstrate that 1 MHz and 5 MHz ultrasound are safe for the neuro-stimulation.

Bottom Line: Electromyography (EMG) collected from tail muscles together with the motion response videos were analyzed for evaluating the stimulation effects.Our results indicate that 5 MHz ultrasound can successfully achieve neuro-stimulation.It provides a smaller stimulation region, which offers improved anatomical specificity for neuro-stimulation in a non-invasive manner.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

ABSTRACT
Low frequency ultrasound (<1 MHz) has been demonstrated to be a promising approach for non-invasive neuro-stimulation. However, the focal width is limited to be half centimeter scale. Minimizing the stimulation region with higher frequency ultrasound will provide a great opportunity to expand its application. This study first time examines the feasibility of using high frequency (5 MHz) ultrasound to achieve neuro-stimulation in brain, and verifies the anatomical specificity of neuro-stimulation in vivo. 1 MHz and 5 MHz ultrasound stimulation were evaluated in the same group of mice. Electromyography (EMG) collected from tail muscles together with the motion response videos were analyzed for evaluating the stimulation effects. Our results indicate that 5 MHz ultrasound can successfully achieve neuro-stimulation. The equivalent diameter (ED) of the stimulation region with 5 MHz ultrasound (0.29 ± 0.08 mm) is significantly smaller than that with 1 MHz (0.83 ± 0.11 mm). The response latency of 5 MHz ultrasound (45 ± 31 ms) is also shorter than that of 1 MHz ultrasound (208 ± 111 ms). Consequently, high frequency (5 MHz) ultrasound can successfully activate the brain circuits in mice. It provides a smaller stimulation region, which offers improved anatomical specificity for neuro-stimulation in a non-invasive manner.

No MeSH data available.