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Nanodroplet-Vaporization-Assisted Sonoporation for Highly Effective Delivery of Photothermal Treatment.

Liu WW, Liu SW, Liou YR, Wu YH, Yang YC, Wang CR, Li PC - Sci Rep (2016)

Bottom Line: This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles.Enhanced cavitation also leads to significant enhancement of the sonoporation effects.Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

View Article: PubMed Central - PubMed

Affiliation: National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, Taipei 106, Taiwan.

ABSTRACT
Sonoporation refers to the use of ultrasound and acoustic cavitation to temporarily enhance the permeability of cellular membranes so as to enhance the delivery efficiency of therapeutic agents into cells. Microbubble-based ultrasound contrast agents are often used to facilitate these cavitation effects. This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles. Significant enhancements were demonstrated both in vitro and in vivo by using gold nanorods encapsulated in nanodroplets for implementing plasmonic photothermal therapy. Combined excitation by ultrasound and laser radiation is used to trigger the gold nanodroplets to induce a liquid-to-gas phase change, which induces cavitation effects that are three-to-fivefold stronger than when using conventional microbubbles. Enhanced cavitation also leads to significant enhancement of the sonoporation effects. Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

No MeSH data available.


Related in: MedlinePlus

Histological investigation.(a) Tumor without any treatment. (b) Magnified image of the area indicated by the rectangle in (a). (c,f) Tumors after being treated with AuNDs-assisted PPTT for either 5 minutes or 2 days. (d,e,g,h) Magnified images of the areas indicated by the solid and dotted-dashed rectangles in (c,f).
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f8: Histological investigation.(a) Tumor without any treatment. (b) Magnified image of the area indicated by the rectangle in (a). (c,f) Tumors after being treated with AuNDs-assisted PPTT for either 5 minutes or 2 days. (d,e,g,h) Magnified images of the areas indicated by the solid and dotted-dashed rectangles in (c,f).

Mentions: To examine the histological damage induced by AuNDs-assisted PPTT on a tumor, tumors treated with AuNDs followed by PPTT for 5 minutes or 2 days and tumors that did not receive AuNDs treatment were dissected out and stained with hematoxylin and eosin (H&E) for histological assessments. Microscopy investigations revealed the creation of several bubble-like structures along with hemorrhage and karyorrhexis (arrows in Fig. 8a–e) in AuNDs-treated tumor tissue after PPTT for 5 minutes. In contrast, tumors that did not receive any treatment did not show any bubble-like structure, hemorrhage, or karyorrhexis. Moreover, after PPTT for 2 days, heat-induced coagulative necrosis that displayed hemorrhage, pyknosis, and karyolysis was found in AuNDs-treated tumor tissue (Fig. 8f). The magnified image in Fig. 8g shows that some hollow spaces were created in the damaged areas, suggesting that coagulative necrotic tumor cell corpses had been cleared. These data show that coagulative necrosis resulting from thermal ablation is the main reason for the death of tumor cells after treatment with AuNDs followed by PPTT.


Nanodroplet-Vaporization-Assisted Sonoporation for Highly Effective Delivery of Photothermal Treatment.

Liu WW, Liu SW, Liou YR, Wu YH, Yang YC, Wang CR, Li PC - Sci Rep (2016)

Histological investigation.(a) Tumor without any treatment. (b) Magnified image of the area indicated by the rectangle in (a). (c,f) Tumors after being treated with AuNDs-assisted PPTT for either 5 minutes or 2 days. (d,e,g,h) Magnified images of the areas indicated by the solid and dotted-dashed rectangles in (c,f).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837361&req=5

f8: Histological investigation.(a) Tumor without any treatment. (b) Magnified image of the area indicated by the rectangle in (a). (c,f) Tumors after being treated with AuNDs-assisted PPTT for either 5 minutes or 2 days. (d,e,g,h) Magnified images of the areas indicated by the solid and dotted-dashed rectangles in (c,f).
Mentions: To examine the histological damage induced by AuNDs-assisted PPTT on a tumor, tumors treated with AuNDs followed by PPTT for 5 minutes or 2 days and tumors that did not receive AuNDs treatment were dissected out and stained with hematoxylin and eosin (H&E) for histological assessments. Microscopy investigations revealed the creation of several bubble-like structures along with hemorrhage and karyorrhexis (arrows in Fig. 8a–e) in AuNDs-treated tumor tissue after PPTT for 5 minutes. In contrast, tumors that did not receive any treatment did not show any bubble-like structure, hemorrhage, or karyorrhexis. Moreover, after PPTT for 2 days, heat-induced coagulative necrosis that displayed hemorrhage, pyknosis, and karyolysis was found in AuNDs-treated tumor tissue (Fig. 8f). The magnified image in Fig. 8g shows that some hollow spaces were created in the damaged areas, suggesting that coagulative necrotic tumor cell corpses had been cleared. These data show that coagulative necrosis resulting from thermal ablation is the main reason for the death of tumor cells after treatment with AuNDs followed by PPTT.

Bottom Line: This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles.Enhanced cavitation also leads to significant enhancement of the sonoporation effects.Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

View Article: PubMed Central - PubMed

Affiliation: National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, Taipei 106, Taiwan.

ABSTRACT
Sonoporation refers to the use of ultrasound and acoustic cavitation to temporarily enhance the permeability of cellular membranes so as to enhance the delivery efficiency of therapeutic agents into cells. Microbubble-based ultrasound contrast agents are often used to facilitate these cavitation effects. This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles. Significant enhancements were demonstrated both in vitro and in vivo by using gold nanorods encapsulated in nanodroplets for implementing plasmonic photothermal therapy. Combined excitation by ultrasound and laser radiation is used to trigger the gold nanodroplets to induce a liquid-to-gas phase change, which induces cavitation effects that are three-to-fivefold stronger than when using conventional microbubbles. Enhanced cavitation also leads to significant enhancement of the sonoporation effects. Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

No MeSH data available.


Related in: MedlinePlus