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Nanodroplet-Vaporization-Assisted Sonoporation for Highly Effective Delivery of Photothermal Treatment.

Liu WW, Liu SW, Liou YR, Wu YH, Yang YC, Wang CR, Li PC - Sci Rep (2016)

Bottom Line: This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles.Enhanced cavitation also leads to significant enhancement of the sonoporation effects.Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

View Article: PubMed Central - PubMed

Affiliation: National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, Taipei 106, Taiwan.

ABSTRACT
Sonoporation refers to the use of ultrasound and acoustic cavitation to temporarily enhance the permeability of cellular membranes so as to enhance the delivery efficiency of therapeutic agents into cells. Microbubble-based ultrasound contrast agents are often used to facilitate these cavitation effects. This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles. Significant enhancements were demonstrated both in vitro and in vivo by using gold nanorods encapsulated in nanodroplets for implementing plasmonic photothermal therapy. Combined excitation by ultrasound and laser radiation is used to trigger the gold nanodroplets to induce a liquid-to-gas phase change, which induces cavitation effects that are three-to-fivefold stronger than when using conventional microbubbles. Enhanced cavitation also leads to significant enhancement of the sonoporation effects. Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

No MeSH data available.


Related in: MedlinePlus

Vaporization of AuNDs.B-mode images showing the temporal profile of the vaporization of AuNDs during exposure to ultrasound only, laser radiation only, ultrasound plus laser radiation, or AuNRs exposed to laser radiation only at 0 minutes, 1.5 minutes, and 3 minutes (left, middle, and right panels). The two white near-vertical linear areas in each image correspond to the walls of the tubes.
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f2: Vaporization of AuNDs.B-mode images showing the temporal profile of the vaporization of AuNDs during exposure to ultrasound only, laser radiation only, ultrasound plus laser radiation, or AuNRs exposed to laser radiation only at 0 minutes, 1.5 minutes, and 3 minutes (left, middle, and right panels). The two white near-vertical linear areas in each image correspond to the walls of the tubes.

Mentions: The vaporization of DDFC AuNDs by optical stimulation generates strong acoustic signals which can be used for ultrasound contrast enhancement36. Thus, we used B-mode imaging to monitor the vaporization of AuNDs (Fig. 2). The two white near-vertical linear areas in each B-mode image correspond to the walls of the tubes, and the presence of AuNDs vaporization is indicated by white areas between the tube walls. The efficacy of AuNDs vaporization was highest for the laser-exposed tube, while the vaporization was barely present in the ultrasound-exposed one. It was also found that even when the vaporization was triggered by the laser radiation and ultrasound concurrently, it was not as obvious as when it was triggered only by the laser radiation. This indicates that the vaporization was triggered mainly by the laser radiation rather than by the ultrasound.


Nanodroplet-Vaporization-Assisted Sonoporation for Highly Effective Delivery of Photothermal Treatment.

Liu WW, Liu SW, Liou YR, Wu YH, Yang YC, Wang CR, Li PC - Sci Rep (2016)

Vaporization of AuNDs.B-mode images showing the temporal profile of the vaporization of AuNDs during exposure to ultrasound only, laser radiation only, ultrasound plus laser radiation, or AuNRs exposed to laser radiation only at 0 minutes, 1.5 minutes, and 3 minutes (left, middle, and right panels). The two white near-vertical linear areas in each image correspond to the walls of the tubes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837361&req=5

f2: Vaporization of AuNDs.B-mode images showing the temporal profile of the vaporization of AuNDs during exposure to ultrasound only, laser radiation only, ultrasound plus laser radiation, or AuNRs exposed to laser radiation only at 0 minutes, 1.5 minutes, and 3 minutes (left, middle, and right panels). The two white near-vertical linear areas in each image correspond to the walls of the tubes.
Mentions: The vaporization of DDFC AuNDs by optical stimulation generates strong acoustic signals which can be used for ultrasound contrast enhancement36. Thus, we used B-mode imaging to monitor the vaporization of AuNDs (Fig. 2). The two white near-vertical linear areas in each B-mode image correspond to the walls of the tubes, and the presence of AuNDs vaporization is indicated by white areas between the tube walls. The efficacy of AuNDs vaporization was highest for the laser-exposed tube, while the vaporization was barely present in the ultrasound-exposed one. It was also found that even when the vaporization was triggered by the laser radiation and ultrasound concurrently, it was not as obvious as when it was triggered only by the laser radiation. This indicates that the vaporization was triggered mainly by the laser radiation rather than by the ultrasound.

Bottom Line: This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles.Enhanced cavitation also leads to significant enhancement of the sonoporation effects.Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

View Article: PubMed Central - PubMed

Affiliation: National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, Taipei 106, Taiwan.

ABSTRACT
Sonoporation refers to the use of ultrasound and acoustic cavitation to temporarily enhance the permeability of cellular membranes so as to enhance the delivery efficiency of therapeutic agents into cells. Microbubble-based ultrasound contrast agents are often used to facilitate these cavitation effects. This study used nanodroplets to significantly enhance the effectiveness of sonoporation relative to using conventional microbubbles. Significant enhancements were demonstrated both in vitro and in vivo by using gold nanorods encapsulated in nanodroplets for implementing plasmonic photothermal therapy. Combined excitation by ultrasound and laser radiation is used to trigger the gold nanodroplets to induce a liquid-to-gas phase change, which induces cavitation effects that are three-to-fivefold stronger than when using conventional microbubbles. Enhanced cavitation also leads to significant enhancement of the sonoporation effects. Our in vivo results show that nanodroplet-vaporization-assisted sonoporation can increase the treatment temperature by more than 10 °C above that achieved by microbubble-based sonoporation.

No MeSH data available.


Related in: MedlinePlus