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Genome-wide identification and expression analysis of the IQD gene family in moso bamboo (Phyllostachys edulis).

Wu M, Li Y, Chen D, Liu H, Zhu D, Xiang Y - Sci Rep (2016)

Bottom Line: We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes.The expression profiles of the IQD genes shed light on their functional divergence.Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

ABSTRACT
Members of the plant-specific IQ67-domain (IQD) protein family are involved in various aspects of normal plant growth and developmental processes as well as basal defence response. Although hundreds of IQD proteins have been identified, only a small number of IQDs have been functionally characterized. Moreover, no systematic study has been performed on moso bamboo. In this study, we performed for the first time a genome-wide identification and expression analysis of the IQD gene family in moso bamboo. We identified 29 non-redundant PeIQD encoding genes. Analysis of the evolutionary patterns and divergence revealed that the IQD genes underwent a large-scale event around 12 million years ago and the division times of IQD family genes between moso bamboo and rice, and, between moso bamboo and Brachypodium, were found to be 20-35 MYA and 25-40 MYA, respectively. We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes. The expression profiles of the IQD genes shed light on their functional divergence. Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

No MeSH data available.


The interaction between PeCaM2 and PeIQD8, PeIQD8Del112-113 or PeIQD8I112T.Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T. (A) pGBKT7-PeIQD8 + pGADT7-PeCaM2; (B) pGBTKT7- PeIQD8 + pGADT7; (C) pGBKT7-PeIQD8Del112-113 + pGADT7-PeCaM2; (D) PeIQD8Del112-113 + pGADT7; (E) pGBKT7-PeIQD8I112T + pGADT7-PeCaM2; (F) pGBKT7-PeIQD8I112T + pGADT7; (G) pGBKT7-Lam + pGADT7-PeCaM2; (H) pGBKT7-PeIQD8 + pGADT7-T.
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f6: The interaction between PeCaM2 and PeIQD8, PeIQD8Del112-113 or PeIQD8I112T.Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T. (A) pGBKT7-PeIQD8 + pGADT7-PeCaM2; (B) pGBTKT7- PeIQD8 + pGADT7; (C) pGBKT7-PeIQD8Del112-113 + pGADT7-PeCaM2; (D) PeIQD8Del112-113 + pGADT7; (E) pGBKT7-PeIQD8I112T + pGADT7-PeCaM2; (F) pGBKT7-PeIQD8I112T + pGADT7; (G) pGBKT7-Lam + pGADT7-PeCaM2; (H) pGBKT7-PeIQD8 + pGADT7-T.

Mentions: When the recombinant plasmid pGBKT7-PeIQD8Del112-113 and pGBKT7-PeIQD8I112T were transformed into the yeast AH109 strain, the self-activation was lost (Figure S3). From Fig. 6, we can clearly see that only the pGBKT7-PeIQD8 and pGADT7-PeCaM2 were co-transformed into the AH109 yeast strain, the selective agar plates (SD/-Trp/-Leu/-Ade/-His/X-α-Gal) turned blue. However, the yeasts that were co-transformed with pGBKT7-PeIQD8Del112-113 and pGADT7-PeCaM2 or pGBKT7-PeIQD8I112T and pGADT7-PeCaM2 were not able to grow on the selective medium. The results suggested that the IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.


Genome-wide identification and expression analysis of the IQD gene family in moso bamboo (Phyllostachys edulis).

Wu M, Li Y, Chen D, Liu H, Zhu D, Xiang Y - Sci Rep (2016)

The interaction between PeCaM2 and PeIQD8, PeIQD8Del112-113 or PeIQD8I112T.Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T. (A) pGBKT7-PeIQD8 + pGADT7-PeCaM2; (B) pGBTKT7- PeIQD8 + pGADT7; (C) pGBKT7-PeIQD8Del112-113 + pGADT7-PeCaM2; (D) PeIQD8Del112-113 + pGADT7; (E) pGBKT7-PeIQD8I112T + pGADT7-PeCaM2; (F) pGBKT7-PeIQD8I112T + pGADT7; (G) pGBKT7-Lam + pGADT7-PeCaM2; (H) pGBKT7-PeIQD8 + pGADT7-T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837358&req=5

f6: The interaction between PeCaM2 and PeIQD8, PeIQD8Del112-113 or PeIQD8I112T.Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T. (A) pGBKT7-PeIQD8 + pGADT7-PeCaM2; (B) pGBTKT7- PeIQD8 + pGADT7; (C) pGBKT7-PeIQD8Del112-113 + pGADT7-PeCaM2; (D) PeIQD8Del112-113 + pGADT7; (E) pGBKT7-PeIQD8I112T + pGADT7-PeCaM2; (F) pGBKT7-PeIQD8I112T + pGADT7; (G) pGBKT7-Lam + pGADT7-PeCaM2; (H) pGBKT7-PeIQD8 + pGADT7-T.
Mentions: When the recombinant plasmid pGBKT7-PeIQD8Del112-113 and pGBKT7-PeIQD8I112T were transformed into the yeast AH109 strain, the self-activation was lost (Figure S3). From Fig. 6, we can clearly see that only the pGBKT7-PeIQD8 and pGADT7-PeCaM2 were co-transformed into the AH109 yeast strain, the selective agar plates (SD/-Trp/-Leu/-Ade/-His/X-α-Gal) turned blue. However, the yeasts that were co-transformed with pGBKT7-PeIQD8Del112-113 and pGADT7-PeCaM2 or pGBKT7-PeIQD8I112T and pGADT7-PeCaM2 were not able to grow on the selective medium. The results suggested that the IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

Bottom Line: We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes.The expression profiles of the IQD genes shed light on their functional divergence.Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

ABSTRACT
Members of the plant-specific IQ67-domain (IQD) protein family are involved in various aspects of normal plant growth and developmental processes as well as basal defence response. Although hundreds of IQD proteins have been identified, only a small number of IQDs have been functionally characterized. Moreover, no systematic study has been performed on moso bamboo. In this study, we performed for the first time a genome-wide identification and expression analysis of the IQD gene family in moso bamboo. We identified 29 non-redundant PeIQD encoding genes. Analysis of the evolutionary patterns and divergence revealed that the IQD genes underwent a large-scale event around 12 million years ago and the division times of IQD family genes between moso bamboo and rice, and, between moso bamboo and Brachypodium, were found to be 20-35 MYA and 25-40 MYA, respectively. We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes. The expression profiles of the IQD genes shed light on their functional divergence. Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

No MeSH data available.