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Genome-wide identification and expression analysis of the IQD gene family in moso bamboo (Phyllostachys edulis).

Wu M, Li Y, Chen D, Liu H, Zhu D, Xiang Y - Sci Rep (2016)

Bottom Line: We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes.The expression profiles of the IQD genes shed light on their functional divergence.Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

ABSTRACT
Members of the plant-specific IQ67-domain (IQD) protein family are involved in various aspects of normal plant growth and developmental processes as well as basal defence response. Although hundreds of IQD proteins have been identified, only a small number of IQDs have been functionally characterized. Moreover, no systematic study has been performed on moso bamboo. In this study, we performed for the first time a genome-wide identification and expression analysis of the IQD gene family in moso bamboo. We identified 29 non-redundant PeIQD encoding genes. Analysis of the evolutionary patterns and divergence revealed that the IQD genes underwent a large-scale event around 12 million years ago and the division times of IQD family genes between moso bamboo and rice, and, between moso bamboo and Brachypodium, were found to be 20-35 MYA and 25-40 MYA, respectively. We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes. The expression profiles of the IQD genes shed light on their functional divergence. Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

No MeSH data available.


Related in: MedlinePlus

PeIQDs interact with PeCaM2 in yeast.The bait construct of pGBKT7-PeIQD8 and the prey construct were co-transformed into yeast strain AH109, then examined on SD/-Trp/-Leu and SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates. Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T.
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f5: PeIQDs interact with PeCaM2 in yeast.The bait construct of pGBKT7-PeIQD8 and the prey construct were co-transformed into yeast strain AH109, then examined on SD/-Trp/-Leu and SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates. Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T.

Mentions: Previous studies have reported that IQD1 and IQD20 can interact with CaM in Arabidopsis67. Recently, it was proposed that ZmIQD15 can interact with CaM in vitro18. To attest whether this interaction also occurs in moso bamboo, yeast two-hybrid analysis was adopted to examine the interaction between PeIQDs and PeCaM2. It was verified that expression of BD-PeIQDs and AD-PeCaM2 was not toxic for AH109 yeast cells and that these fusion proteins have no transcriptional activation ability when expressed separately (Figure S3). Our results showed that all the transformants tested were found to grow well on SD/-Leu/-Trp medium. When transferred onto SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates for 5 days, both the positive control and the experimental group turned blue. In contrast, the negative control displayed no α-galactosidase activity (Fig. 5). The results suggested that PeIQDs can interact with PeCaM2 in vitro.


Genome-wide identification and expression analysis of the IQD gene family in moso bamboo (Phyllostachys edulis).

Wu M, Li Y, Chen D, Liu H, Zhu D, Xiang Y - Sci Rep (2016)

PeIQDs interact with PeCaM2 in yeast.The bait construct of pGBKT7-PeIQD8 and the prey construct were co-transformed into yeast strain AH109, then examined on SD/-Trp/-Leu and SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates. Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837358&req=5

f5: PeIQDs interact with PeCaM2 in yeast.The bait construct of pGBKT7-PeIQD8 and the prey construct were co-transformed into yeast strain AH109, then examined on SD/-Trp/-Leu and SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates. Positive control, pGBKT7-53 + pGADT7-T; negative control, pGBKT7-Lam + pGADT7-T.
Mentions: Previous studies have reported that IQD1 and IQD20 can interact with CaM in Arabidopsis67. Recently, it was proposed that ZmIQD15 can interact with CaM in vitro18. To attest whether this interaction also occurs in moso bamboo, yeast two-hybrid analysis was adopted to examine the interaction between PeIQDs and PeCaM2. It was verified that expression of BD-PeIQDs and AD-PeCaM2 was not toxic for AH109 yeast cells and that these fusion proteins have no transcriptional activation ability when expressed separately (Figure S3). Our results showed that all the transformants tested were found to grow well on SD/-Leu/-Trp medium. When transferred onto SD/-Trp/-Leu/-Ade/-His/X-α-Gal plates for 5 days, both the positive control and the experimental group turned blue. In contrast, the negative control displayed no α-galactosidase activity (Fig. 5). The results suggested that PeIQDs can interact with PeCaM2 in vitro.

Bottom Line: We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes.The expression profiles of the IQD genes shed light on their functional divergence.Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

ABSTRACT
Members of the plant-specific IQ67-domain (IQD) protein family are involved in various aspects of normal plant growth and developmental processes as well as basal defence response. Although hundreds of IQD proteins have been identified, only a small number of IQDs have been functionally characterized. Moreover, no systematic study has been performed on moso bamboo. In this study, we performed for the first time a genome-wide identification and expression analysis of the IQD gene family in moso bamboo. We identified 29 non-redundant PeIQD encoding genes. Analysis of the evolutionary patterns and divergence revealed that the IQD genes underwent a large-scale event around 12 million years ago and the division times of IQD family genes between moso bamboo and rice, and, between moso bamboo and Brachypodium, were found to be 20-35 MYA and 25-40 MYA, respectively. We surveyed the putative promoter regions of the PeIQD genes, which showed that largely stress-related cis-elements existed in these genes. The expression profiles of the IQD genes shed light on their functional divergence. Additionally, a yeast two-hybrid assay proved that PeIQD8 can interact with PeCaM2 and that IQ or I in the IQ motif is required for PeIQD8 to combine with CaM2.

No MeSH data available.


Related in: MedlinePlus