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Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Detection of the oxygenation of haemoglobin using SERS spectroscopy.(a) Distribution of value of I1358/I1372 for both groups. (b) Distribution of value of I1606/I1638 for both groups. The light red histogram represented Group 1while the grey histogram represented Group 2. The dark red part is the overlap of both groups. The SERS spectra number brought into statistics of Group 1 is n = 138, and that of Group 2 is n = 156.
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f6: Detection of the oxygenation of haemoglobin using SERS spectroscopy.(a) Distribution of value of I1358/I1372 for both groups. (b) Distribution of value of I1606/I1638 for both groups. The light red histogram represented Group 1while the grey histogram represented Group 2. The dark red part is the overlap of both groups. The SERS spectra number brought into statistics of Group 1 is n = 138, and that of Group 2 is n = 156.

Mentions: Figure 6 presents the statistical distributions of I1358/I1372 and I1606/I1638 for Group 1 (age range of 60–85) and Group 2 (age range of 18–30). Figure 6a showed that the value of I1358/I1372 of the RBCs of Group 1 is mainly distributed in the range of 2.5–6 with the maximum in the range of 3.75–4.25. Meanwhile the value of I1358/I1372 of the RBCs of Group 2 is mainly distributed in the range of 0.5–2 with the maximum in the range of 1.25–1.5. In Fig. 6b, it showed the distribution of the value of I1606/I1638 of Group 1 and Group 2. For RBCs of Group 1, the value of I1606/I1638 is mainly distributed in the range of 1.4–2.0 with the maximum in the range of 1.6–1.7. For RBCs of Group 2, the value of I1606/I1638 is mainly distributed in the range of 0.3–1 with the maximum in the range of 0.6–0.75. As discussed above, these results indicate that the haemoglobin in RBCs obtained from donors in Group 1 tends to be in the T state more than that of the haemoglobin of RBCs isolated from donors in Group 2, which reveals that the level of haemoglobin oxygenation in Group 1 is lower than that in Group 2. Donors in Group 1 are much older than those in Group 2. Therefore, it seems likely that the observed difference of the haemoglobin state between the two groups is because the degree of activity and metabolism of the people in Group 1 will be much lower than those of the people in Group 2. Moreover, older people are more likely to have underlying disease than younger ones, which may also influence the level of haemoglobin oxygenation in their blood samples.


Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Detection of the oxygenation of haemoglobin using SERS spectroscopy.(a) Distribution of value of I1358/I1372 for both groups. (b) Distribution of value of I1606/I1638 for both groups. The light red histogram represented Group 1while the grey histogram represented Group 2. The dark red part is the overlap of both groups. The SERS spectra number brought into statistics of Group 1 is n = 138, and that of Group 2 is n = 156.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837339&req=5

f6: Detection of the oxygenation of haemoglobin using SERS spectroscopy.(a) Distribution of value of I1358/I1372 for both groups. (b) Distribution of value of I1606/I1638 for both groups. The light red histogram represented Group 1while the grey histogram represented Group 2. The dark red part is the overlap of both groups. The SERS spectra number brought into statistics of Group 1 is n = 138, and that of Group 2 is n = 156.
Mentions: Figure 6 presents the statistical distributions of I1358/I1372 and I1606/I1638 for Group 1 (age range of 60–85) and Group 2 (age range of 18–30). Figure 6a showed that the value of I1358/I1372 of the RBCs of Group 1 is mainly distributed in the range of 2.5–6 with the maximum in the range of 3.75–4.25. Meanwhile the value of I1358/I1372 of the RBCs of Group 2 is mainly distributed in the range of 0.5–2 with the maximum in the range of 1.25–1.5. In Fig. 6b, it showed the distribution of the value of I1606/I1638 of Group 1 and Group 2. For RBCs of Group 1, the value of I1606/I1638 is mainly distributed in the range of 1.4–2.0 with the maximum in the range of 1.6–1.7. For RBCs of Group 2, the value of I1606/I1638 is mainly distributed in the range of 0.3–1 with the maximum in the range of 0.6–0.75. As discussed above, these results indicate that the haemoglobin in RBCs obtained from donors in Group 1 tends to be in the T state more than that of the haemoglobin of RBCs isolated from donors in Group 2, which reveals that the level of haemoglobin oxygenation in Group 1 is lower than that in Group 2. Donors in Group 1 are much older than those in Group 2. Therefore, it seems likely that the observed difference of the haemoglobin state between the two groups is because the degree of activity and metabolism of the people in Group 1 will be much lower than those of the people in Group 2. Moreover, older people are more likely to have underlying disease than younger ones, which may also influence the level of haemoglobin oxygenation in their blood samples.

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.