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Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Related in: MedlinePlus

Two categories of SERS spectra of RBCs on the SERS substrate.In (a), intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In (b), intensity of 1638 cm−1 is much higher than that of other characteristic peaks. For both (a) and (b), the red curves are the SERS spectra of RBC on SERS substrate while the black curves are the Raman spectra of RBC on glass slide.
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f5: Two categories of SERS spectra of RBCs on the SERS substrate.In (a), intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In (b), intensity of 1638 cm−1 is much higher than that of other characteristic peaks. For both (a) and (b), the red curves are the SERS spectra of RBC on SERS substrate while the black curves are the Raman spectra of RBC on glass slide.

Mentions: We used the silver nanostructures grown with an external current of 60 μA as SERS substrates for RBCs according to the results in Fig. 4. Since this substrate has marked Raman enhancement, we were able to use a low laser power of just 0.05 mW, which minimized the damage to samples without losing spectral details. Figure 5 shows two categories of SERS spectra of RBCs on SERS substrates. In Fig. 5a the intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In Fig. 5b the intensity of 1638 cm−1 is much higher than that of other characteristic peaks. Raman spectra of RBCs on glass slides were measured under the same conditions as controls. On the SERS substrates, the intensity of the spectrum of RBCs is more than one order of magnitude higher than that on glass slides. Moreover, the SERS spectra of RBCs on SERS substrates contain more peaks and details than the control spectra. In contrast, the peaks in this region of the spectra obtained for RBCs on glass slides are almost masked by noise and difficult to detect. Therefore, our silver nanostructured SERS substrates allow SERS spectral detection of RBCs by 514 nm excitation at low laser power.


Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Two categories of SERS spectra of RBCs on the SERS substrate.In (a), intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In (b), intensity of 1638 cm−1 is much higher than that of other characteristic peaks. For both (a) and (b), the red curves are the SERS spectra of RBC on SERS substrate while the black curves are the Raman spectra of RBC on glass slide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837339&req=5

f5: Two categories of SERS spectra of RBCs on the SERS substrate.In (a), intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In (b), intensity of 1638 cm−1 is much higher than that of other characteristic peaks. For both (a) and (b), the red curves are the SERS spectra of RBC on SERS substrate while the black curves are the Raman spectra of RBC on glass slide.
Mentions: We used the silver nanostructures grown with an external current of 60 μA as SERS substrates for RBCs according to the results in Fig. 4. Since this substrate has marked Raman enhancement, we were able to use a low laser power of just 0.05 mW, which minimized the damage to samples without losing spectral details. Figure 5 shows two categories of SERS spectra of RBCs on SERS substrates. In Fig. 5a the intensity of 1358 cm−1 is much higher than that of other characteristic peaks; In Fig. 5b the intensity of 1638 cm−1 is much higher than that of other characteristic peaks. Raman spectra of RBCs on glass slides were measured under the same conditions as controls. On the SERS substrates, the intensity of the spectrum of RBCs is more than one order of magnitude higher than that on glass slides. Moreover, the SERS spectra of RBCs on SERS substrates contain more peaks and details than the control spectra. In contrast, the peaks in this region of the spectra obtained for RBCs on glass slides are almost masked by noise and difficult to detect. Therefore, our silver nanostructured SERS substrates allow SERS spectral detection of RBCs by 514 nm excitation at low laser power.

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Related in: MedlinePlus