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Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Related in: MedlinePlus

SERS spectra for 10−6 mol L−1 R6G on silver nanostructure substrates grown with different external currents.The spectrum labelled R6G is that of R6G on a glass slide as control.
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f4: SERS spectra for 10−6 mol L−1 R6G on silver nanostructure substrates grown with different external currents.The spectrum labelled R6G is that of R6G on a glass slide as control.

Mentions: R6G was used to examine the enhancement effect of the silver nanostructures as SERS substrates. The results are provided in Fig. 4. The intensity of the SERS spectra of R6G appears rising trend with growth current for the ranges of 5–30 μA and 40–60 μA. At 60 μA the intensity of SERS spectrum reaches highest. When growth current exceeds 60 μA, the intensity of SERS spectra decreased.


Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

SERS spectra for 10−6 mol L−1 R6G on silver nanostructure substrates grown with different external currents.The spectrum labelled R6G is that of R6G on a glass slide as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837339&req=5

f4: SERS spectra for 10−6 mol L−1 R6G on silver nanostructure substrates grown with different external currents.The spectrum labelled R6G is that of R6G on a glass slide as control.
Mentions: R6G was used to examine the enhancement effect of the silver nanostructures as SERS substrates. The results are provided in Fig. 4. The intensity of the SERS spectra of R6G appears rising trend with growth current for the ranges of 5–30 μA and 40–60 μA. At 60 μA the intensity of SERS spectrum reaches highest. When growth current exceeds 60 μA, the intensity of SERS spectra decreased.

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Related in: MedlinePlus