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Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.


Characterization of silver nanostructures grown by the solid-state ionics method.(a) Camera image of the silver nanostructure. (the ~2 mm length denser region below dash line is grown with 5 μA for 6 h; the ~1 cm length sparser region above dash line is grown with 30 μA for 2 h. (b) X-ray EDS and (c) XRD pattern of the silver nanostructure grown with an external current of 30 μA for 2 h.
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f1: Characterization of silver nanostructures grown by the solid-state ionics method.(a) Camera image of the silver nanostructure. (the ~2 mm length denser region below dash line is grown with 5 μA for 6 h; the ~1 cm length sparser region above dash line is grown with 30 μA for 2 h. (b) X-ray EDS and (c) XRD pattern of the silver nanostructure grown with an external current of 30 μA for 2 h.

Mentions: Characterization data for the silver nanostructures grown with an external current of 30 μA for 2 h by the solid-state ionics method are presented in Fig. 1a. The nanostructures cover an area of about 5 cm × 1 cm, which is very rapid growth. In fact, we found that the growth rate of silver nanostructures is proportional to the intensity of the external direct current18. X-ray energy-dispersive spectroscopy (EDS) results (Fig. 1b) showed that the silver nanostructured film is pure silver. The X-ray diffraction (XRD) pattern (Fig. 1c) contained a high-intensity diffraction peak at about 38.20° and other peaks were observed at about 44.42°, 64.40°, and 77.32° corresponding to the (111), (200), (220), and (311) crystalline planes of the cubic crystalline structure of Ag (JCPDS card file no. 4783), respectively.


Rapid, controllable growth of silver nanostructured surface-enhanced Raman scattering substrates for red blood cell detection.

Zhang S, Tian X, Yin J, Liu Y, Dong Z, Sun JL, Ma W - Sci Rep (2016)

Characterization of silver nanostructures grown by the solid-state ionics method.(a) Camera image of the silver nanostructure. (the ~2 mm length denser region below dash line is grown with 5 μA for 6 h; the ~1 cm length sparser region above dash line is grown with 30 μA for 2 h. (b) X-ray EDS and (c) XRD pattern of the silver nanostructure grown with an external current of 30 μA for 2 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4837339&req=5

f1: Characterization of silver nanostructures grown by the solid-state ionics method.(a) Camera image of the silver nanostructure. (the ~2 mm length denser region below dash line is grown with 5 μA for 6 h; the ~1 cm length sparser region above dash line is grown with 30 μA for 2 h. (b) X-ray EDS and (c) XRD pattern of the silver nanostructure grown with an external current of 30 μA for 2 h.
Mentions: Characterization data for the silver nanostructures grown with an external current of 30 μA for 2 h by the solid-state ionics method are presented in Fig. 1a. The nanostructures cover an area of about 5 cm × 1 cm, which is very rapid growth. In fact, we found that the growth rate of silver nanostructures is proportional to the intensity of the external direct current18. X-ray energy-dispersive spectroscopy (EDS) results (Fig. 1b) showed that the silver nanostructured film is pure silver. The X-ray diffraction (XRD) pattern (Fig. 1c) contained a high-intensity diffraction peak at about 38.20° and other peaks were observed at about 44.42°, 64.40°, and 77.32° corresponding to the (111), (200), (220), and (311) crystalline planes of the cubic crystalline structure of Ag (JCPDS card file no. 4783), respectively.

Bottom Line: A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors.This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people.Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Huazhong Agricultural University, 430070, Wuhan, China.

ABSTRACT
Silver nanostructured films suitable for use as surface-enhanced Raman scattering (SERS) substrates are prepared in just 2 hours by the solid-state ionics method. By changing the intensity of the external direct current, we can readily control the surface morphology and growth rate of the silver nanostructured films. A detailed investigation of the surface enhancement of the silver nanostructured films using Rhodamine 6G (R6G) as a molecular probe revealed that the enhancement factor of the films was up to 10(11). We used the silver nanostructured films as substrates in SERS detection of human red blood cells (RBCs). The SERS spectra of RBCs on the silver nanostructured film could be clearly detected at a laser power of just 0.05 mW. Comparison of the SERS spectra of RBCs obtained from younger and older donors showed that the SERS spectra depended on donor age. A greater proportion of the haemoglobin in the RBCs of older donors was in the deoxygenated state than that of the younger donors. This implies that haemoglobin of older people has lower oxygen-carrying capacity than that of younger people. Overall, the fabricated silver substrates show promise in biomedical SERS spectral detection.

No MeSH data available.