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Association of three putative periodontal pathogens with chronic periodontitis in Brazilian subjects.

Gonçalves C, Soares GM, Faveri M, Pérez-Chaparro PJ, Lobão E, Figueiredo LC, Baccelli GT, Feres M - J Appl Oral Sci (2016)

Bottom Line: Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test).We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test).The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Periodontia, Universidade de Guarulhos, Guarulhos, SP, Brasil.

ABSTRACT
Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.

No MeSH data available.


Related in: MedlinePlus

Mean counts (x105) of the three bacterial species evaluated. The Mann-Whitney test was used to assess significance of differences between groups; **p<0.01 and ***p<0.001
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f02: Mean counts (x105) of the three bacterial species evaluated. The Mann-Whitney test was used to assess significance of differences between groups; **p<0.01 and ***p<0.001

Mentions: Figures 1 and 2 present the mean percentage of sites colonized by F. alocis, D. pneumosintes and P. endodontalis and the mean counts of these species, respectively, in the two clinical groups. All three species were present in a statistically significant, higher percentage of sites and mean counts in subjects with ChP than in those in PH (p<0.05). The mean levels of F. alocis, D. pneumosintes and P. endodontalis in the healthy subjects were 0.01x105, 0.12x105 and 1.89x105, respectively; and 1.77x105, 7.61x105 and 4.01x105, respectively, in the periodontitis subjects. Figure 3 shows the mean counts (x105) of the three microbial species in subjects with PH and in sites with PD ≥5 mm and ≤4 mm in subjects with ChP. F. alocis and D. pneumosintes were present in statistically significant, higher mean levels in shallow and deep sites of periodontitis subjects in comparison with the levels observed in healthy subjects (p<0.05). The levels of P. endodontalis did not differ significantly between shallow sites of subjects with ChP and PH.


Association of three putative periodontal pathogens with chronic periodontitis in Brazilian subjects.

Gonçalves C, Soares GM, Faveri M, Pérez-Chaparro PJ, Lobão E, Figueiredo LC, Baccelli GT, Feres M - J Appl Oral Sci (2016)

Mean counts (x105) of the three bacterial species evaluated. The Mann-Whitney test was used to assess significance of differences between groups; **p<0.01 and ***p<0.001
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836926&req=5

f02: Mean counts (x105) of the three bacterial species evaluated. The Mann-Whitney test was used to assess significance of differences between groups; **p<0.01 and ***p<0.001
Mentions: Figures 1 and 2 present the mean percentage of sites colonized by F. alocis, D. pneumosintes and P. endodontalis and the mean counts of these species, respectively, in the two clinical groups. All three species were present in a statistically significant, higher percentage of sites and mean counts in subjects with ChP than in those in PH (p<0.05). The mean levels of F. alocis, D. pneumosintes and P. endodontalis in the healthy subjects were 0.01x105, 0.12x105 and 1.89x105, respectively; and 1.77x105, 7.61x105 and 4.01x105, respectively, in the periodontitis subjects. Figure 3 shows the mean counts (x105) of the three microbial species in subjects with PH and in sites with PD ≥5 mm and ≤4 mm in subjects with ChP. F. alocis and D. pneumosintes were present in statistically significant, higher mean levels in shallow and deep sites of periodontitis subjects in comparison with the levels observed in healthy subjects (p<0.05). The levels of P. endodontalis did not differ significantly between shallow sites of subjects with ChP and PH.

Bottom Line: Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test).We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test).The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Periodontia, Universidade de Guarulhos, Guarulhos, SP, Brasil.

ABSTRACT
Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.

No MeSH data available.


Related in: MedlinePlus