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Evaluation of an experimental rat model for comparative studies of bleaching agents.

Cintra LT, Benetti F, Ferreira LL, Rahal V, Ervolino E, Jacinto Rde C, Gomes Filho JE, Briso AL - J Appl Oral Sci (2016)

Bottom Line: Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05).In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas.Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Endodontia, Faculdade de Odontologia de Araçatuba, Univ. Estadual Paulista, Araçatuba, SP, Brasil.

ABSTRACT
Dental materials in general are tested in different animal models prior to the clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents, by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats' vital teeth. Material and Methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals were untreated (control). The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated through histomorphometric analysis with inflammatory cell count in the coronal and radicular thirds of the pulp. Fibroblasts were also counted. Scores were attributed to odontoblastic layer and vascular changes. Tertiary dentin area and pulp chamber central area were measured histomorphometrically. Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05). Results After 2 days, the amount of inflammatory cells increased in the coronal pulp occlusal third up to the 15-min application groups of each bleaching gel. In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, reduction on the pulp chamber central area and enlargement of the tertiary dentin area were observed, without the detection of inflammation areas. Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.

No MeSH data available.


Related in: MedlinePlus

Central area measurement of the pulp chamber in the experimental groups using the Leica QWin V3 Image Processing and Analysis Software (Leica Microsystems, Wetzlar, Hesse, Germany). The values obtained were analyzed by the Kolmogorov-Smirnov normality test and the one-way analysis of variance (p<0.05)
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f01: Central area measurement of the pulp chamber in the experimental groups using the Leica QWin V3 Image Processing and Analysis Software (Leica Microsystems, Wetzlar, Hesse, Germany). The values obtained were analyzed by the Kolmogorov-Smirnov normality test and the one-way analysis of variance (p<0.05)

Mentions: The mean central area of the pulp chamber was measured by image processing software (Leica QWin V3, Leica Microsystems, Wetzlar, Hesse, Germany) (Figure 1). With the values obtained, it was possible to calculate the percentage reduction in the central area of the pulp chamber in the treated groups, considering the central area of the control group.


Evaluation of an experimental rat model for comparative studies of bleaching agents.

Cintra LT, Benetti F, Ferreira LL, Rahal V, Ervolino E, Jacinto Rde C, Gomes Filho JE, Briso AL - J Appl Oral Sci (2016)

Central area measurement of the pulp chamber in the experimental groups using the Leica QWin V3 Image Processing and Analysis Software (Leica Microsystems, Wetzlar, Hesse, Germany). The values obtained were analyzed by the Kolmogorov-Smirnov normality test and the one-way analysis of variance (p<0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836925&req=5

f01: Central area measurement of the pulp chamber in the experimental groups using the Leica QWin V3 Image Processing and Analysis Software (Leica Microsystems, Wetzlar, Hesse, Germany). The values obtained were analyzed by the Kolmogorov-Smirnov normality test and the one-way analysis of variance (p<0.05)
Mentions: The mean central area of the pulp chamber was measured by image processing software (Leica QWin V3, Leica Microsystems, Wetzlar, Hesse, Germany) (Figure 1). With the values obtained, it was possible to calculate the percentage reduction in the central area of the pulp chamber in the treated groups, considering the central area of the control group.

Bottom Line: Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05).In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas.Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Endodontia, Faculdade de Odontologia de Araçatuba, Univ. Estadual Paulista, Araçatuba, SP, Brasil.

ABSTRACT
Dental materials in general are tested in different animal models prior to the clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents, by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats' vital teeth. Material and Methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals were untreated (control). The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated through histomorphometric analysis with inflammatory cell count in the coronal and radicular thirds of the pulp. Fibroblasts were also counted. Scores were attributed to odontoblastic layer and vascular changes. Tertiary dentin area and pulp chamber central area were measured histomorphometrically. Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05). Results After 2 days, the amount of inflammatory cells increased in the coronal pulp occlusal third up to the 15-min application groups of each bleaching gel. In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, reduction on the pulp chamber central area and enlargement of the tertiary dentin area were observed, without the detection of inflammation areas. Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.

No MeSH data available.


Related in: MedlinePlus