Limits...
Effects of a buried magnetic field on cranial bone reconstruction in rats.

Abreu MC, Ponzoni D, Langie R, Artuzi FE, Puricelli E - J Appl Oral Sci (2016)

Bottom Line: Results These analyses showed significant group by postoperative time interactions (p=0.008).Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days.Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Oral e Maxilofacial, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

ABSTRACT
The understanding of bone repair phenomena is a fundamental part of dentistry and maxillofacial surgery. Objective The present study aimed to evaluate the influence of buried magnetic field stimulation on bone repair in rat calvaria after reconstruction with autogenous bone grafts, synthetic powdered hydroxyapatite, or allogeneic cartilage grafts, with or without exposure to magnetic stimulation. Material and Methods Ninety male Wistar rats were divided into 18 groups of five animals each. Critical bone defects were created in the rats' calvaria and immediately reconstructed with autogenous bone, powdered synthetic hydroxyapatite or allogeneic cartilage. Magnetic implants were also placed in half the animals. Rats were euthanized for analysis at 15, 30, and 60 postoperative days. Histomorphometric analyses of the quantity of bone repair were performed at all times. Results These analyses showed significant group by postoperative time interactions (p=0.008). Among the rats subjected to autogenous bone reconstruction, those exposed to magnetic stimulation had higher bone fill percentages than those without magnetic implants. Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days. Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

No MeSH data available.


Related in: MedlinePlus

Mosaic-like arrangement of the entire length of the bone defect and central portion of the defect. Superior image: BDL indicates the bone defect limit, DC indicates position of the metal device used (magnet or titanium disk), BD indicates the bone defect area; Inferior image: it shows the image obtained for histomorphometric analysis after exclusion of areas outside the critical bone defect created; the black line delimits the autogenous bone graft and the green line delimits the new bone
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836924&req=5

f02: Mosaic-like arrangement of the entire length of the bone defect and central portion of the defect. Superior image: BDL indicates the bone defect limit, DC indicates position of the metal device used (magnet or titanium disk), BD indicates the bone defect area; Inferior image: it shows the image obtained for histomorphometric analysis after exclusion of areas outside the critical bone defect created; the black line delimits the autogenous bone graft and the green line delimits the new bone

Mentions: The histological field examined included the entire length of the bone defect, and 100X-magnified images of the histological slides were captured by an Olympus® video camera (Model 5, Qcolor Cooler, RTV) coupled to a binocular microscope (Olympus Optical Co.®, CX41RF) and a Dell computer (Dimension 5150) running Qcapture® software (version 2.81; Quantitative Imaging Corporation, Inc.; 2005). Photomicrographs were grouped side by side in a mosaic-like arrangement to allow for the visualization and measurement of the total area of the bone defect. Subsequently, the external images of the bone defect were deleted, and only the images of the central portion of the defect were used for analysis (Figure 2).


Effects of a buried magnetic field on cranial bone reconstruction in rats.

Abreu MC, Ponzoni D, Langie R, Artuzi FE, Puricelli E - J Appl Oral Sci (2016)

Mosaic-like arrangement of the entire length of the bone defect and central portion of the defect. Superior image: BDL indicates the bone defect limit, DC indicates position of the metal device used (magnet or titanium disk), BD indicates the bone defect area; Inferior image: it shows the image obtained for histomorphometric analysis after exclusion of areas outside the critical bone defect created; the black line delimits the autogenous bone graft and the green line delimits the new bone
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836924&req=5

f02: Mosaic-like arrangement of the entire length of the bone defect and central portion of the defect. Superior image: BDL indicates the bone defect limit, DC indicates position of the metal device used (magnet or titanium disk), BD indicates the bone defect area; Inferior image: it shows the image obtained for histomorphometric analysis after exclusion of areas outside the critical bone defect created; the black line delimits the autogenous bone graft and the green line delimits the new bone
Mentions: The histological field examined included the entire length of the bone defect, and 100X-magnified images of the histological slides were captured by an Olympus® video camera (Model 5, Qcolor Cooler, RTV) coupled to a binocular microscope (Olympus Optical Co.®, CX41RF) and a Dell computer (Dimension 5150) running Qcapture® software (version 2.81; Quantitative Imaging Corporation, Inc.; 2005). Photomicrographs were grouped side by side in a mosaic-like arrangement to allow for the visualization and measurement of the total area of the bone defect. Subsequently, the external images of the bone defect were deleted, and only the images of the central portion of the defect were used for analysis (Figure 2).

Bottom Line: Results These analyses showed significant group by postoperative time interactions (p=0.008).Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days.Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Oral e Maxilofacial, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

ABSTRACT
The understanding of bone repair phenomena is a fundamental part of dentistry and maxillofacial surgery. Objective The present study aimed to evaluate the influence of buried magnetic field stimulation on bone repair in rat calvaria after reconstruction with autogenous bone grafts, synthetic powdered hydroxyapatite, or allogeneic cartilage grafts, with or without exposure to magnetic stimulation. Material and Methods Ninety male Wistar rats were divided into 18 groups of five animals each. Critical bone defects were created in the rats' calvaria and immediately reconstructed with autogenous bone, powdered synthetic hydroxyapatite or allogeneic cartilage. Magnetic implants were also placed in half the animals. Rats were euthanized for analysis at 15, 30, and 60 postoperative days. Histomorphometric analyses of the quantity of bone repair were performed at all times. Results These analyses showed significant group by postoperative time interactions (p=0.008). Among the rats subjected to autogenous bone reconstruction, those exposed to magnetic stimulation had higher bone fill percentages than those without magnetic implants. Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days. Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

No MeSH data available.


Related in: MedlinePlus