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Effects of a buried magnetic field on cranial bone reconstruction in rats.

Abreu MC, Ponzoni D, Langie R, Artuzi FE, Puricelli E - J Appl Oral Sci (2016)

Bottom Line: Results These analyses showed significant group by postoperative time interactions (p=0.008).Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days.Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Oral e Maxilofacial, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

ABSTRACT
The understanding of bone repair phenomena is a fundamental part of dentistry and maxillofacial surgery. Objective The present study aimed to evaluate the influence of buried magnetic field stimulation on bone repair in rat calvaria after reconstruction with autogenous bone grafts, synthetic powdered hydroxyapatite, or allogeneic cartilage grafts, with or without exposure to magnetic stimulation. Material and Methods Ninety male Wistar rats were divided into 18 groups of five animals each. Critical bone defects were created in the rats' calvaria and immediately reconstructed with autogenous bone, powdered synthetic hydroxyapatite or allogeneic cartilage. Magnetic implants were also placed in half the animals. Rats were euthanized for analysis at 15, 30, and 60 postoperative days. Histomorphometric analyses of the quantity of bone repair were performed at all times. Results These analyses showed significant group by postoperative time interactions (p=0.008). Among the rats subjected to autogenous bone reconstruction, those exposed to magnetic stimulation had higher bone fill percentages than those without magnetic implants. Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days. Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

No MeSH data available.


Related in: MedlinePlus

Reconstruction of critical bone defects in rat calvaria using different materials. a) Dimensions of critical bone defects; b) Reconstruction with autogenous bone graft; c) Reconstruction with powdered synthetic hydroxyapatite implant; d) Reconstruction with allogeneic cartilage graft
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f01: Reconstruction of critical bone defects in rat calvaria using different materials. a) Dimensions of critical bone defects; b) Reconstruction with autogenous bone graft; c) Reconstruction with powdered synthetic hydroxyapatite implant; d) Reconstruction with allogeneic cartilage graft

Mentions: Strict asepsis was observed during the procedures. The rats were anesthetized by intraperitoneal ketamine hydrochloride (100 mg/kg) and xylazine hydrochloride (10 mg/kg), as well as local bupivacaine (2 mg/kg). A trephine drill (Neodent®, Curitiba, PR, Brazil) was used to create a bicortical defect in the frontal bone, measuring 5 mm in diameter and 1 mm in depth. Two 2 mm osteotomies with 1 mm gaps were created anterior and posterior to the bone defect for the placement of the two magnets. This method was used to facilitate the penetration of the magnetic field in the bone defect. The defect was filled with each of the different materials (Figure 1). During the postoperative period, the rats received food and water, and 5 mg/kg Tramadol for pain relief. Animals were euthanized by decapitation after the previously described postoperative periods.


Effects of a buried magnetic field on cranial bone reconstruction in rats.

Abreu MC, Ponzoni D, Langie R, Artuzi FE, Puricelli E - J Appl Oral Sci (2016)

Reconstruction of critical bone defects in rat calvaria using different materials. a) Dimensions of critical bone defects; b) Reconstruction with autogenous bone graft; c) Reconstruction with powdered synthetic hydroxyapatite implant; d) Reconstruction with allogeneic cartilage graft
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836924&req=5

f01: Reconstruction of critical bone defects in rat calvaria using different materials. a) Dimensions of critical bone defects; b) Reconstruction with autogenous bone graft; c) Reconstruction with powdered synthetic hydroxyapatite implant; d) Reconstruction with allogeneic cartilage graft
Mentions: Strict asepsis was observed during the procedures. The rats were anesthetized by intraperitoneal ketamine hydrochloride (100 mg/kg) and xylazine hydrochloride (10 mg/kg), as well as local bupivacaine (2 mg/kg). A trephine drill (Neodent®, Curitiba, PR, Brazil) was used to create a bicortical defect in the frontal bone, measuring 5 mm in diameter and 1 mm in depth. Two 2 mm osteotomies with 1 mm gaps were created anterior and posterior to the bone defect for the placement of the two magnets. This method was used to facilitate the penetration of the magnetic field in the bone defect. The defect was filled with each of the different materials (Figure 1). During the postoperative period, the rats received food and water, and 5 mg/kg Tramadol for pain relief. Animals were euthanized by decapitation after the previously described postoperative periods.

Bottom Line: Results These analyses showed significant group by postoperative time interactions (p=0.008).Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days.Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Oral e Maxilofacial, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

ABSTRACT
The understanding of bone repair phenomena is a fundamental part of dentistry and maxillofacial surgery. Objective The present study aimed to evaluate the influence of buried magnetic field stimulation on bone repair in rat calvaria after reconstruction with autogenous bone grafts, synthetic powdered hydroxyapatite, or allogeneic cartilage grafts, with or without exposure to magnetic stimulation. Material and Methods Ninety male Wistar rats were divided into 18 groups of five animals each. Critical bone defects were created in the rats' calvaria and immediately reconstructed with autogenous bone, powdered synthetic hydroxyapatite or allogeneic cartilage. Magnetic implants were also placed in half the animals. Rats were euthanized for analysis at 15, 30, and 60 postoperative days. Histomorphometric analyses of the quantity of bone repair were performed at all times. Results These analyses showed significant group by postoperative time interactions (p=0.008). Among the rats subjected to autogenous bone reconstruction, those exposed to magnetic stimulation had higher bone fill percentages than those without magnetic implants. Results also showed that the quality of bone repair remained higher in the former group as compared to the latter at 60 postoperative days. Conclusions After 60 postoperative days, bone repair was greater in the group treated with autogenous bone grafts and exposed to a magnetic field, and bone repair was most pronounced in animals treated with autogenous bone grafts, followed by those treated with powdered synthetic hydroxyapatite and allogeneic cartilage grafts.

No MeSH data available.


Related in: MedlinePlus