Limits...
Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis.

Rahman MZ, Shigeishi H, Sasaki K, Ota A, Ohta K, Takechi M - J Appl Oral Sci (2016)

Bottom Line: The present in vitro study was undertaken to examine the relationship between these two bioactive agents and their combinatory effects on osteoblastic activity and mineralization in vitro.The synergy of the proliferative potential of FGF-2 and the differentiation potential of MEL showed increased osteogenic activity in MC3T3-E1 cells cultured within IP-CHA constructs.Conclusion These findings indicate that the combination of FGF-2 and MEL may be utilized with biocompatible materials to attain augmented osteogenic activity and mineralization.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

ABSTRACT
Objective Biocompatible materials such as interconnected porous hydroxyapatite ceramics (IP-CHA) loaded with osteogenic cells and bioactive agents are part of an evolving concept for overcoming craniofacial defects by use of artificial bone tissue regeneration. Amongst the bioactive agents, melatonin (MEL) and basic fibroblast growth factor (FGF-2) have been independently reported to induce osteoblastic activity. The present in vitro study was undertaken to examine the relationship between these two bioactive agents and their combinatory effects on osteoblastic activity and mineralization in vitro. Material and Methods Mouse preosteoblast cells (MC3T3-E1) were seeded and cultured within cylindrical type of IP-CHA block (ø 4x7 mm) by vacuum-assisted method. The IP-CHA/MC3T3 composites were subjected to FGF-2 and/or MEL. The proliferation assay, alkaline phosphatase enzyme activity (ALP), mRNA expressions of late bone markers, namely Osteocalcin (OCN) and Osteopontin (OPN), and Alizarin Red staining were examined over a period of 7 days. Results FGF-2 mainly enhanced the proliferation of MC3T3-E1 cells within the IP-CHA constructs. MEL mainly induced the mRNA expression of late bone markers (OCN and OPN) and showed increased ALP activity of MC3T3 cells cultured within IP-CHA construct. Moreover, the combination of FGF-2 and MEL showed increased osteogenic activity within the IP-CHA construct in terms of cell proliferation, upregulated expressions of OCN and OPN, increased ALP activity and mineralization with Alizarin Red. The synergy of the proliferative potential of FGF-2 and the differentiation potential of MEL showed increased osteogenic activity in MC3T3-E1 cells cultured within IP-CHA constructs. Conclusion These findings indicate that the combination of FGF-2 and MEL may be utilized with biocompatible materials to attain augmented osteogenic activity and mineralization.

No MeSH data available.


Related in: MedlinePlus

Proliferation assay of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). Cell growth was significantly induced by FGF-2 treatment compared to the control and to the treatment with MEL. The combination of MEL and FGF-2 more markedly promoted the proliferation of IP-CHA/MC3T3-E1 cells. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836923&req=5

f04: Proliferation assay of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). Cell growth was significantly induced by FGF-2 treatment compared to the control and to the treatment with MEL. The combination of MEL and FGF-2 more markedly promoted the proliferation of IP-CHA/MC3T3-E1 cells. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively

Mentions: To evaluate the combined effect of FGF-2 and MEL on the proliferative potential of MC3T3-E1 cells within an IP-CHA construct, IP-CHA/MC3T3-E1 composites were examined in the presence of 20 µg/ml FGF-2 and/or 200 nM MEL after 1, 3, and 5 days of culture. FGF-2 independently and significantly induced growth of MC3T3-E1 cells compared to the control on day 5 (Figure 4), whereas MEL alone showed no significant effect on cell proliferation.


Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis.

Rahman MZ, Shigeishi H, Sasaki K, Ota A, Ohta K, Takechi M - J Appl Oral Sci (2016)

Proliferation assay of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). Cell growth was significantly induced by FGF-2 treatment compared to the control and to the treatment with MEL. The combination of MEL and FGF-2 more markedly promoted the proliferation of IP-CHA/MC3T3-E1 cells. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836923&req=5

f04: Proliferation assay of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). Cell growth was significantly induced by FGF-2 treatment compared to the control and to the treatment with MEL. The combination of MEL and FGF-2 more markedly promoted the proliferation of IP-CHA/MC3T3-E1 cells. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively
Mentions: To evaluate the combined effect of FGF-2 and MEL on the proliferative potential of MC3T3-E1 cells within an IP-CHA construct, IP-CHA/MC3T3-E1 composites were examined in the presence of 20 µg/ml FGF-2 and/or 200 nM MEL after 1, 3, and 5 days of culture. FGF-2 independently and significantly induced growth of MC3T3-E1 cells compared to the control on day 5 (Figure 4), whereas MEL alone showed no significant effect on cell proliferation.

Bottom Line: The present in vitro study was undertaken to examine the relationship between these two bioactive agents and their combinatory effects on osteoblastic activity and mineralization in vitro.The synergy of the proliferative potential of FGF-2 and the differentiation potential of MEL showed increased osteogenic activity in MC3T3-E1 cells cultured within IP-CHA constructs.Conclusion These findings indicate that the combination of FGF-2 and MEL may be utilized with biocompatible materials to attain augmented osteogenic activity and mineralization.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

ABSTRACT
Objective Biocompatible materials such as interconnected porous hydroxyapatite ceramics (IP-CHA) loaded with osteogenic cells and bioactive agents are part of an evolving concept for overcoming craniofacial defects by use of artificial bone tissue regeneration. Amongst the bioactive agents, melatonin (MEL) and basic fibroblast growth factor (FGF-2) have been independently reported to induce osteoblastic activity. The present in vitro study was undertaken to examine the relationship between these two bioactive agents and their combinatory effects on osteoblastic activity and mineralization in vitro. Material and Methods Mouse preosteoblast cells (MC3T3-E1) were seeded and cultured within cylindrical type of IP-CHA block (ø 4x7 mm) by vacuum-assisted method. The IP-CHA/MC3T3 composites were subjected to FGF-2 and/or MEL. The proliferation assay, alkaline phosphatase enzyme activity (ALP), mRNA expressions of late bone markers, namely Osteocalcin (OCN) and Osteopontin (OPN), and Alizarin Red staining were examined over a period of 7 days. Results FGF-2 mainly enhanced the proliferation of MC3T3-E1 cells within the IP-CHA constructs. MEL mainly induced the mRNA expression of late bone markers (OCN and OPN) and showed increased ALP activity of MC3T3 cells cultured within IP-CHA construct. Moreover, the combination of FGF-2 and MEL showed increased osteogenic activity within the IP-CHA construct in terms of cell proliferation, upregulated expressions of OCN and OPN, increased ALP activity and mineralization with Alizarin Red. The synergy of the proliferative potential of FGF-2 and the differentiation potential of MEL showed increased osteogenic activity in MC3T3-E1 cells cultured within IP-CHA constructs. Conclusion These findings indicate that the combination of FGF-2 and MEL may be utilized with biocompatible materials to attain augmented osteogenic activity and mineralization.

No MeSH data available.


Related in: MedlinePlus