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Sealing ability of MTA, CPM, and MBPc as root-end filling materials: a bacterial leakage study.

Medeiros PL, Bernardineli N, Cavenago BC, Torres SA, Duarte MA, Bramante CM, Marciano MA - J Appl Oral Sci (2016)

Bottom Line: The MBPc showed significantly less bacterial leakage compared with the MTA group (p<0.05).No significant differences were found between the CPM and the other groups.Conclusions The epoxy resin-based cement MBPc had lower bacterial leakage compared with the calcium silicate-based cements MTA and CPM.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.

ABSTRACT
Objectives To evaluate the sealing ability of three root-end filling materials (white MTA, CPM, and MBPc) using an Enterococcus faecalis leakage model. Material and Methods Seventy single-root extracted human teeth were instrumented and root-ends were resected to prepare 3 mm depth cavities. Root-end preparations were filled with white MTA, CPM, and MBPc cements. Enterococcus faecalis was coronally introduced and the apical portion was immersed in BHI culture medium with phenol red indicator. The bacterial leakage was monitored every 24 h for 4 weeks. The statistical analysis was performed using the Wilcoxon-Gehan test (p<0.05). Results All cements showed bacterial leakage after 24 hours, except for the negative control group. The MBPc showed significantly less bacterial leakage compared with the MTA group (p<0.05). No significant differences were found between the CPM and the other groups. Conclusions The epoxy resin-based cement MBPc had lower bacterial leakage compared with the calcium silicate-based cements MTA and CPM.

No MeSH data available.


Related in: MedlinePlus

Representative leakage apparatus used for the experiment. Negative microbial growth is shown in A and positive microbial growth can be observed in B
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f02: Representative leakage apparatus used for the experiment. Negative microbial growth is shown in A and positive microbial growth can be observed in B

Mentions: The apparatus used to evaluate the leakage was prepared as previously described21. Glass vials with rubber stoppers were adjusted to use. Using a high-speed handpiece, a hole was made through the centre of each rubber stopper in which each tooth was inserted under a pressure up to 4 mm from the apical portion. An epoxy resin-based varnish (Araldite, Brascola, São Paulo, São Paulo, Brazil) was used to seal the interface between tooth and rubber stopper. Cylinders prepared with 10 mL plastic syringes were adapted to the outer surface of the stoppers to create a chamber around the crown of the tooth. The apparatus was sterilized in ethylene oxide gas for a 4 h cycle at 56°C (Figure 2).


Sealing ability of MTA, CPM, and MBPc as root-end filling materials: a bacterial leakage study.

Medeiros PL, Bernardineli N, Cavenago BC, Torres SA, Duarte MA, Bramante CM, Marciano MA - J Appl Oral Sci (2016)

Representative leakage apparatus used for the experiment. Negative microbial growth is shown in A and positive microbial growth can be observed in B
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836922&req=5

f02: Representative leakage apparatus used for the experiment. Negative microbial growth is shown in A and positive microbial growth can be observed in B
Mentions: The apparatus used to evaluate the leakage was prepared as previously described21. Glass vials with rubber stoppers were adjusted to use. Using a high-speed handpiece, a hole was made through the centre of each rubber stopper in which each tooth was inserted under a pressure up to 4 mm from the apical portion. An epoxy resin-based varnish (Araldite, Brascola, São Paulo, São Paulo, Brazil) was used to seal the interface between tooth and rubber stopper. Cylinders prepared with 10 mL plastic syringes were adapted to the outer surface of the stoppers to create a chamber around the crown of the tooth. The apparatus was sterilized in ethylene oxide gas for a 4 h cycle at 56°C (Figure 2).

Bottom Line: The MBPc showed significantly less bacterial leakage compared with the MTA group (p<0.05).No significant differences were found between the CPM and the other groups.Conclusions The epoxy resin-based cement MBPc had lower bacterial leakage compared with the calcium silicate-based cements MTA and CPM.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.

ABSTRACT
Objectives To evaluate the sealing ability of three root-end filling materials (white MTA, CPM, and MBPc) using an Enterococcus faecalis leakage model. Material and Methods Seventy single-root extracted human teeth were instrumented and root-ends were resected to prepare 3 mm depth cavities. Root-end preparations were filled with white MTA, CPM, and MBPc cements. Enterococcus faecalis was coronally introduced and the apical portion was immersed in BHI culture medium with phenol red indicator. The bacterial leakage was monitored every 24 h for 4 weeks. The statistical analysis was performed using the Wilcoxon-Gehan test (p<0.05). Results All cements showed bacterial leakage after 24 hours, except for the negative control group. The MBPc showed significantly less bacterial leakage compared with the MTA group (p<0.05). No significant differences were found between the CPM and the other groups. Conclusions The epoxy resin-based cement MBPc had lower bacterial leakage compared with the calcium silicate-based cements MTA and CPM.

No MeSH data available.


Related in: MedlinePlus