Limits...
Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes.

Binta B, Patel M - J Appl Oral Sci (2016)

Bottom Line: The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp.Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics.Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.

View Article: PubMed Central - PubMed

Affiliation: School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Gauteng, South Africa.

ABSTRACT
Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. Presumptive β-lactamase-producing isolates were evaluated for definite β-lactamase production using the nitrocefin slide method and identified using the API Rapid 32A system. Antimicrobial susceptibility was performed using disc diffusion and microbroth dilution tests as described by CLSI Methods. Isolates were screened for the presence of the β-lactamase-TEM (BlaTEM) and β-lactamase-cfxA genes using Polymerase Chain Reaction (PCR). Amplified PCR products were sequenced and the cfxA gene was characterized using Genbank databases. Results Seventy five percent of patients carried two species of β-lactamase-producing anaerobic bacteria that comprised 9.4% of the total number of cultivable bacteria. Fifty one percent of β-lactamase-producing strains mainly Prevotella, Porphyromonas, and Bacteroides carried the cfxA gene, whereas none of them carried blaTEM. Further characterization of the cfxA gene showed that 76.7% of these strains carried the cfxA2 gene, 14% carried cfxA3, and 9.3% carried cfxA6. The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp. Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics. Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.

No MeSH data available.


Related in: MedlinePlus

β-lactamase-producing oral anaerobic bacteria
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836921&req=5

f01: β-lactamase-producing oral anaerobic bacteria

Mentions: The mean age of the patients was 52 with a range from 32 to 83 years of age. Fifty-eight percent of the patients were female. The average pocket depth of the sampled pockets was 7 mm with a range of 5 mm to 13 mm. Mean total anaerobic bacterial count on control plate was 1.8x106 cfu/mL. Mean total anaerobic bacterial count on the amoxicillin plate was 1.9x105 cfu/mL. Mean total anaerobic bacterial count on the augmentin plate was 5.9x104 cfu/mL. Seventy-five percent of patients carried on average two species of β-lactamase-producing anaerobic oral bacteria, which constitute 9.4% of the total cultivable number of bacteria. All the isolates that grew on the blood agar plate containing amoxicillin tested positive for β-lactamase with the nitrocefin test. Seventy-eight of the 85 isolates of β-lactamase-producing bacteria were mainly gram negative black pigmented anaerobes (Figure 1).


Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes.

Binta B, Patel M - J Appl Oral Sci (2016)

β-lactamase-producing oral anaerobic bacteria
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836921&req=5

f01: β-lactamase-producing oral anaerobic bacteria
Mentions: The mean age of the patients was 52 with a range from 32 to 83 years of age. Fifty-eight percent of the patients were female. The average pocket depth of the sampled pockets was 7 mm with a range of 5 mm to 13 mm. Mean total anaerobic bacterial count on control plate was 1.8x106 cfu/mL. Mean total anaerobic bacterial count on the amoxicillin plate was 1.9x105 cfu/mL. Mean total anaerobic bacterial count on the augmentin plate was 5.9x104 cfu/mL. Seventy-five percent of patients carried on average two species of β-lactamase-producing anaerobic oral bacteria, which constitute 9.4% of the total cultivable number of bacteria. All the isolates that grew on the blood agar plate containing amoxicillin tested positive for β-lactamase with the nitrocefin test. Seventy-eight of the 85 isolates of β-lactamase-producing bacteria were mainly gram negative black pigmented anaerobes (Figure 1).

Bottom Line: The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp.Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics.Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.

View Article: PubMed Central - PubMed

Affiliation: School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Gauteng, South Africa.

ABSTRACT
Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. Presumptive β-lactamase-producing isolates were evaluated for definite β-lactamase production using the nitrocefin slide method and identified using the API Rapid 32A system. Antimicrobial susceptibility was performed using disc diffusion and microbroth dilution tests as described by CLSI Methods. Isolates were screened for the presence of the β-lactamase-TEM (BlaTEM) and β-lactamase-cfxA genes using Polymerase Chain Reaction (PCR). Amplified PCR products were sequenced and the cfxA gene was characterized using Genbank databases. Results Seventy five percent of patients carried two species of β-lactamase-producing anaerobic bacteria that comprised 9.4% of the total number of cultivable bacteria. Fifty one percent of β-lactamase-producing strains mainly Prevotella, Porphyromonas, and Bacteroides carried the cfxA gene, whereas none of them carried blaTEM. Further characterization of the cfxA gene showed that 76.7% of these strains carried the cfxA2 gene, 14% carried cfxA3, and 9.3% carried cfxA6. The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp. Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics. Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.

No MeSH data available.


Related in: MedlinePlus