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Impact of Opium Addiction on Levels of Pro- and Anti-inflammatory Cytokines after Surgery.

Lashkarizadeh MR, Garshasbi M, Shabani M, Dabiri S, Hadavi H, Manafi-Anari H - Addict Health (2016)

Bottom Line: Then, cytokines were measured before surgery, immediately after surgery (within 30-60 minutes) and 24 hours after surgery.IFN-γ was raised in an addicted group in three phases of the study as compared to that of the control group.The results revealed that opium addiction can increase plasma level of IFN-γ in rats and decrease plasma level of IL-4 after surgical stress.

View Article: PubMed Central - PubMed

Affiliation: Associate Professor, Department of Surgery, School of Medicine AND Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Opium addiction alters immune responses to stresses such as an injury due to changing the secretion of cytokines. The present study assessed the effect of opium addiction on the cytokines [tumor necrosis factor α (TNFα), interferon-γ (IFN-γ), interleukin-4 (IL-4), and IL-10] before and after laparotomy.

Methods: Male rats were randomly divided into control and opium addicted (n = 20). Then, cytokines were measured before surgery, immediately after surgery (within 30-60 minutes) and 24 hours after surgery.

Findings: IFN-γ was raised in an addicted group in three phases of the study as compared to that of the control group. IL-4 in opium addicted group decreased in two phases after surgery compared to the control group. IL-4 was lower after surgery in comparison to before surgery in the opium addicted group. The difference in IL-10 and TNFα levels was not statistically significant in the all groups measured in three phases of the investigation.

Conclusion: The results revealed that opium addiction can increase plasma level of IFN-γ in rats and decrease plasma level of IL-4 after surgical stress. It seems that opium addicted rats are a more susceptible to increased inflammation.

No MeSH data available.


Related in: MedlinePlus

Serum levels of the tumor necrosis factor α(TNFα) in opium addicted versus control groupThere was not statistically different in TNFα level measuredin before surgery, immediately after surgery and 24 hoursafter surgery between two groups
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f2-AHJ-08-009: Serum levels of the tumor necrosis factor α(TNFα) in opium addicted versus control groupThere was not statistically different in TNFα level measuredin before surgery, immediately after surgery and 24 hoursafter surgery between two groups

Mentions: The results showed that the difference in the measured serum level of IL-10 (Figure 1) and TNFα (Figure 2) in the two groups (addicted vs. control), in all phases of the investigation (before and after surgery) was not statistically significant (P > 0.050). During the all phases of examination serum level of IFN-γ (Figure 3) increased in addicted group compared to control group (P < 0.001). Serum level of IL-4 (Figure 4) in the opium addicted group decreased after surgery compared to before surgery (P = 0.006) as well as to the control group (within 30-60 minutes after surgery: P < 0.010; 24 hours after surgery: P < 0.050) (Figure 4). In control group, no difference was found in the measured serum cytokines level after surgery compared to the measured levels before surgery (P > 0.050).


Impact of Opium Addiction on Levels of Pro- and Anti-inflammatory Cytokines after Surgery.

Lashkarizadeh MR, Garshasbi M, Shabani M, Dabiri S, Hadavi H, Manafi-Anari H - Addict Health (2016)

Serum levels of the tumor necrosis factor α(TNFα) in opium addicted versus control groupThere was not statistically different in TNFα level measuredin before surgery, immediately after surgery and 24 hoursafter surgery between two groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836758&req=5

f2-AHJ-08-009: Serum levels of the tumor necrosis factor α(TNFα) in opium addicted versus control groupThere was not statistically different in TNFα level measuredin before surgery, immediately after surgery and 24 hoursafter surgery between two groups
Mentions: The results showed that the difference in the measured serum level of IL-10 (Figure 1) and TNFα (Figure 2) in the two groups (addicted vs. control), in all phases of the investigation (before and after surgery) was not statistically significant (P > 0.050). During the all phases of examination serum level of IFN-γ (Figure 3) increased in addicted group compared to control group (P < 0.001). Serum level of IL-4 (Figure 4) in the opium addicted group decreased after surgery compared to before surgery (P = 0.006) as well as to the control group (within 30-60 minutes after surgery: P < 0.010; 24 hours after surgery: P < 0.050) (Figure 4). In control group, no difference was found in the measured serum cytokines level after surgery compared to the measured levels before surgery (P > 0.050).

Bottom Line: Then, cytokines were measured before surgery, immediately after surgery (within 30-60 minutes) and 24 hours after surgery.IFN-γ was raised in an addicted group in three phases of the study as compared to that of the control group.The results revealed that opium addiction can increase plasma level of IFN-γ in rats and decrease plasma level of IL-4 after surgical stress.

View Article: PubMed Central - PubMed

Affiliation: Associate Professor, Department of Surgery, School of Medicine AND Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Opium addiction alters immune responses to stresses such as an injury due to changing the secretion of cytokines. The present study assessed the effect of opium addiction on the cytokines [tumor necrosis factor α (TNFα), interferon-γ (IFN-γ), interleukin-4 (IL-4), and IL-10] before and after laparotomy.

Methods: Male rats were randomly divided into control and opium addicted (n = 20). Then, cytokines were measured before surgery, immediately after surgery (within 30-60 minutes) and 24 hours after surgery.

Findings: IFN-γ was raised in an addicted group in three phases of the study as compared to that of the control group. IL-4 in opium addicted group decreased in two phases after surgery compared to the control group. IL-4 was lower after surgery in comparison to before surgery in the opium addicted group. The difference in IL-10 and TNFα levels was not statistically significant in the all groups measured in three phases of the investigation.

Conclusion: The results revealed that opium addiction can increase plasma level of IFN-γ in rats and decrease plasma level of IL-4 after surgical stress. It seems that opium addicted rats are a more susceptible to increased inflammation.

No MeSH data available.


Related in: MedlinePlus