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Two Groups of Thellungiella salsuginea RAVs Exhibit Distinct Responses and Sensitivity to Salt and ABA in Transgenic Arabidopsis.

Yang S, Luo C, Song Y, Wang J - PLoS ONE (2016)

Bottom Line: Under normal conditions, the germination process of all TsRAVs overexpressing transgenic seeds was inhibited with a stronger effect observed in 35S:A-TsRAVs seeds than in 35S:B-TsRAVs seeds.All 35S:TsRAVs transgenic plants showed a similar degree of reduction in root growth compared with untreated seedlings in the presence of ABA.Taken together, our results suggest that two groups of TsRAVs perform distinct regulating roles during plant growth and abiotic defense including drought and salt, and A-TsRAVs are more likely than B-TsRAVs to act as negative regulators in the above-mentioned biological processes.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.

ABSTRACT
Containing both AP2 domain and B3 domain, RAV (Related to ABI3/VP1) transcription factors are involved in diverse functions in higher plants. A total of eight TsRAV genes were isolated from the genome of Thellungiella salsuginea and could be divided into two groups (A- and B-group) based on their sequence similarity. The mRNA abundance of all Thellungiella salsuginea TsRAVs followed a gradual decline during seed germination. In Thellungiella salsuginea seedling, transcripts of TsRAVs in the group A (A-TsRAVs) were gradually and moderately reduced by salt treatment but rapidly and severely repressed by ABA treatment. In comparison, with a barely detectable constitutive expression, the transcriptional level of TsRAVs in the group B (B-TsRAVs) exhibited a moderate induction in cotyledons when confronted with ABA. We then produced the "gain-of-function" transgenic Arabidopsis plants for each TsRAV gene and found that only 35S:A-TsRAVs showed weak growth retardation including reduced root elongation, suggesting their roles in negatively controlling plant growth. Under normal conditions, the germination process of all TsRAVs overexpressing transgenic seeds was inhibited with a stronger effect observed in 35S:A-TsRAVs seeds than in 35S:B-TsRAVs seeds. With the presence of NaCl, seed germination and seedling root elongation of all plants including wild type and 35S:TsRAVs plants were retarded and a more severe inhibition occurred to the 35S:A-TsRAV transgenic plants. ABA treatment only negatively affected the germination rates of 35S:A-TsRAV transgenic seeds but not those of 35S:B-TsRAV transgenic seeds. All 35S:TsRAVs transgenic plants showed a similar degree of reduction in root growth compared with untreated seedlings in the presence of ABA. Furthermore, the cotyledon greening/expansion was more severely inhibited 35S:A-TsRAVs than in 35S:B-TsRAVs seedlings. Upon water deficiency, with a wider opening of stomata, 35S:A-TsRAVs plants experienced a faster transpirational water loss than wild type and 35S:B-TsRAVs lines. Taken together, our results suggest that two groups of TsRAVs perform distinct regulating roles during plant growth and abiotic defense including drought and salt, and A-TsRAVs are more likely than B-TsRAVs to act as negative regulators in the above-mentioned biological processes.

No MeSH data available.


Related in: MedlinePlus

Phenotypic characterization of 35S:TsRAVs transgenic Arabidopsis plants under normal conditions.(A) Primary root length of 7-d-old 35S:TsRAVs transgenic Arabidopsis seedlings grown on 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 50 seedlings were measured for each replicates. Different letters indicate significant differences among means (P<0.05 by Tukey’s test). (B) Germination rates of 35S:TsRAVs transgenic Arabidopsis seeds during a 5-day period on normal 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 100 seeds were measured in each replicated.
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pone.0153517.g003: Phenotypic characterization of 35S:TsRAVs transgenic Arabidopsis plants under normal conditions.(A) Primary root length of 7-d-old 35S:TsRAVs transgenic Arabidopsis seedlings grown on 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 50 seedlings were measured for each replicates. Different letters indicate significant differences among means (P<0.05 by Tukey’s test). (B) Germination rates of 35S:TsRAVs transgenic Arabidopsis seeds during a 5-day period on normal 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 100 seeds were measured in each replicated.

Mentions: To understand the involvement of TsRAV genes in response to stressed conditions, we generated the “gain-of-function” transgenic lines by introducing each of the eight TsRAV genes individually into Arabidopsis under the control of the CaMV 35S promoter. For each TsRAV transgene, three homozygous overexpressing (OE) lines with the highest transgene expression level were selected for further analysis (S1 Fig). The previously reported inhibitory effects of AtRAV1 and AtRAV2 on plant growth [7,34,40,41] supported their roles as negative regulators during Arabidopsis growth and development [1]. In this work, we observed severe root growth retardation in 35S:A-TsRAVs transgenic lines but not in 35S:B-TsRAVs lines (Fig 3A). The only morphological defect detected in the vegetative and floral tissues was the narrower and longer leaves shown by 35S:TsRAV7 transgenic plants (S2 Fig).


Two Groups of Thellungiella salsuginea RAVs Exhibit Distinct Responses and Sensitivity to Salt and ABA in Transgenic Arabidopsis.

Yang S, Luo C, Song Y, Wang J - PLoS ONE (2016)

Phenotypic characterization of 35S:TsRAVs transgenic Arabidopsis plants under normal conditions.(A) Primary root length of 7-d-old 35S:TsRAVs transgenic Arabidopsis seedlings grown on 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 50 seedlings were measured for each replicates. Different letters indicate significant differences among means (P<0.05 by Tukey’s test). (B) Germination rates of 35S:TsRAVs transgenic Arabidopsis seeds during a 5-day period on normal 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 100 seeds were measured in each replicated.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836749&req=5

pone.0153517.g003: Phenotypic characterization of 35S:TsRAVs transgenic Arabidopsis plants under normal conditions.(A) Primary root length of 7-d-old 35S:TsRAVs transgenic Arabidopsis seedlings grown on 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 50 seedlings were measured for each replicates. Different letters indicate significant differences among means (P<0.05 by Tukey’s test). (B) Germination rates of 35S:TsRAVs transgenic Arabidopsis seeds during a 5-day period on normal 1/2 MS media. Each data bar represents the means ± SE of three replicates. More than 100 seeds were measured in each replicated.
Mentions: To understand the involvement of TsRAV genes in response to stressed conditions, we generated the “gain-of-function” transgenic lines by introducing each of the eight TsRAV genes individually into Arabidopsis under the control of the CaMV 35S promoter. For each TsRAV transgene, three homozygous overexpressing (OE) lines with the highest transgene expression level were selected for further analysis (S1 Fig). The previously reported inhibitory effects of AtRAV1 and AtRAV2 on plant growth [7,34,40,41] supported their roles as negative regulators during Arabidopsis growth and development [1]. In this work, we observed severe root growth retardation in 35S:A-TsRAVs transgenic lines but not in 35S:B-TsRAVs lines (Fig 3A). The only morphological defect detected in the vegetative and floral tissues was the narrower and longer leaves shown by 35S:TsRAV7 transgenic plants (S2 Fig).

Bottom Line: Under normal conditions, the germination process of all TsRAVs overexpressing transgenic seeds was inhibited with a stronger effect observed in 35S:A-TsRAVs seeds than in 35S:B-TsRAVs seeds.All 35S:TsRAVs transgenic plants showed a similar degree of reduction in root growth compared with untreated seedlings in the presence of ABA.Taken together, our results suggest that two groups of TsRAVs perform distinct regulating roles during plant growth and abiotic defense including drought and salt, and A-TsRAVs are more likely than B-TsRAVs to act as negative regulators in the above-mentioned biological processes.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.

ABSTRACT
Containing both AP2 domain and B3 domain, RAV (Related to ABI3/VP1) transcription factors are involved in diverse functions in higher plants. A total of eight TsRAV genes were isolated from the genome of Thellungiella salsuginea and could be divided into two groups (A- and B-group) based on their sequence similarity. The mRNA abundance of all Thellungiella salsuginea TsRAVs followed a gradual decline during seed germination. In Thellungiella salsuginea seedling, transcripts of TsRAVs in the group A (A-TsRAVs) were gradually and moderately reduced by salt treatment but rapidly and severely repressed by ABA treatment. In comparison, with a barely detectable constitutive expression, the transcriptional level of TsRAVs in the group B (B-TsRAVs) exhibited a moderate induction in cotyledons when confronted with ABA. We then produced the "gain-of-function" transgenic Arabidopsis plants for each TsRAV gene and found that only 35S:A-TsRAVs showed weak growth retardation including reduced root elongation, suggesting their roles in negatively controlling plant growth. Under normal conditions, the germination process of all TsRAVs overexpressing transgenic seeds was inhibited with a stronger effect observed in 35S:A-TsRAVs seeds than in 35S:B-TsRAVs seeds. With the presence of NaCl, seed germination and seedling root elongation of all plants including wild type and 35S:TsRAVs plants were retarded and a more severe inhibition occurred to the 35S:A-TsRAV transgenic plants. ABA treatment only negatively affected the germination rates of 35S:A-TsRAV transgenic seeds but not those of 35S:B-TsRAV transgenic seeds. All 35S:TsRAVs transgenic plants showed a similar degree of reduction in root growth compared with untreated seedlings in the presence of ABA. Furthermore, the cotyledon greening/expansion was more severely inhibited 35S:A-TsRAVs than in 35S:B-TsRAVs seedlings. Upon water deficiency, with a wider opening of stomata, 35S:A-TsRAVs plants experienced a faster transpirational water loss than wild type and 35S:B-TsRAVs lines. Taken together, our results suggest that two groups of TsRAVs perform distinct regulating roles during plant growth and abiotic defense including drought and salt, and A-TsRAVs are more likely than B-TsRAVs to act as negative regulators in the above-mentioned biological processes.

No MeSH data available.


Related in: MedlinePlus