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Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus

Comparisons of serum sex steroids at 36 days.The concentrations of estrone (A), estradiol (B), androstenedione (C) and testosterone (D) in the serum were quantified with ELISA kit. These data were shown as Mean±sem. * p < 0.05 versus control group, # p < 0.05 versus PCOS group.
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pone.0153196.g005: Comparisons of serum sex steroids at 36 days.The concentrations of estrone (A), estradiol (B), androstenedione (C) and testosterone (D) in the serum were quantified with ELISA kit. These data were shown as Mean±sem. * p < 0.05 versus control group, # p < 0.05 versus PCOS group.

Mentions: The serum estradiol and estrone concentrations were significantly lower in PCOS rats than those in the control rats. When PCOS rats were treated with FMT or Lactobacillus, the estradiol and estrone levels were increased significantly compared with those in PCOS rats (Fig 5A and 5B). FMT was more effective in elevating estradiol and estrone levels than Lactobacillus transplantation as there was no significant difference between the FMT group and the control group.


Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Comparisons of serum sex steroids at 36 days.The concentrations of estrone (A), estradiol (B), androstenedione (C) and testosterone (D) in the serum were quantified with ELISA kit. These data were shown as Mean±sem. * p < 0.05 versus control group, # p < 0.05 versus PCOS group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836746&req=5

pone.0153196.g005: Comparisons of serum sex steroids at 36 days.The concentrations of estrone (A), estradiol (B), androstenedione (C) and testosterone (D) in the serum were quantified with ELISA kit. These data were shown as Mean±sem. * p < 0.05 versus control group, # p < 0.05 versus PCOS group.
Mentions: The serum estradiol and estrone concentrations were significantly lower in PCOS rats than those in the control rats. When PCOS rats were treated with FMT or Lactobacillus, the estradiol and estrone levels were increased significantly compared with those in PCOS rats (Fig 5A and 5B). FMT was more effective in elevating estradiol and estrone levels than Lactobacillus transplantation as there was no significant difference between the FMT group and the control group.

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus