Limits...
Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus

Morphological changes of ovarian tissues.(A) Sections of ovary from the control group had normal appearance. (B) PCOS group showed cystic degenerating follicles with thin granulosa layers. (C) PCOS Lactobacillus transplantation group showed increased granulosa layers. (D) PCOS FMT group showed increased granulosa layers and formation of corpora lutea. The larger boxed area in A, B, C, D was shown at higher magnification (400×) in E, F, G, H, respectively. GLC: granular cell layer, TCL: theca cell layer, O: oocyte, L: luteum, AF: atretic follicle. Magnification (100×), scale bar, 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836746&req=5

pone.0153196.g004: Morphological changes of ovarian tissues.(A) Sections of ovary from the control group had normal appearance. (B) PCOS group showed cystic degenerating follicles with thin granulosa layers. (C) PCOS Lactobacillus transplantation group showed increased granulosa layers. (D) PCOS FMT group showed increased granulosa layers and formation of corpora lutea. The larger boxed area in A, B, C, D was shown at higher magnification (400×) in E, F, G, H, respectively. GLC: granular cell layer, TCL: theca cell layer, O: oocyte, L: luteum, AF: atretic follicle. Magnification (100×), scale bar, 50 μm.

Mentions: Under light microscopy, the control rat ovaries exhibited follicles in various stages of development including secondary follicles, graafian follicles, and fresh corpora lutea. The granulosa within the follicles showed multiple layers. The PCOS rat ovaries showed small follicles in early stage of development and atretic follicles. In addition, many large cysts with virtually no granulosa layer or scant granulosa were observed. The FMT and Lactobacillus transplantation rat ovaries showed increased granulosa layers and the formation of corpora lutea (Fig 4 and Table 3).


Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Morphological changes of ovarian tissues.(A) Sections of ovary from the control group had normal appearance. (B) PCOS group showed cystic degenerating follicles with thin granulosa layers. (C) PCOS Lactobacillus transplantation group showed increased granulosa layers. (D) PCOS FMT group showed increased granulosa layers and formation of corpora lutea. The larger boxed area in A, B, C, D was shown at higher magnification (400×) in E, F, G, H, respectively. GLC: granular cell layer, TCL: theca cell layer, O: oocyte, L: luteum, AF: atretic follicle. Magnification (100×), scale bar, 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836746&req=5

pone.0153196.g004: Morphological changes of ovarian tissues.(A) Sections of ovary from the control group had normal appearance. (B) PCOS group showed cystic degenerating follicles with thin granulosa layers. (C) PCOS Lactobacillus transplantation group showed increased granulosa layers. (D) PCOS FMT group showed increased granulosa layers and formation of corpora lutea. The larger boxed area in A, B, C, D was shown at higher magnification (400×) in E, F, G, H, respectively. GLC: granular cell layer, TCL: theca cell layer, O: oocyte, L: luteum, AF: atretic follicle. Magnification (100×), scale bar, 50 μm.
Mentions: Under light microscopy, the control rat ovaries exhibited follicles in various stages of development including secondary follicles, graafian follicles, and fresh corpora lutea. The granulosa within the follicles showed multiple layers. The PCOS rat ovaries showed small follicles in early stage of development and atretic follicles. In addition, many large cysts with virtually no granulosa layer or scant granulosa were observed. The FMT and Lactobacillus transplantation rat ovaries showed increased granulosa layers and the formation of corpora lutea (Fig 4 and Table 3).

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus