Limits...
Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus

Estrous cycle changes in three representative rats from each group.Cycle stages are as follows: 1, diestrus; 2, proestrus; 3, estrus and 4, metestrus. Groups are as follows: (A) Control group; (B) PCOS group; (C) PCOS Lactobacillus transplantation group; (D) PCOS FMT group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836746&req=5

pone.0153196.g003: Estrous cycle changes in three representative rats from each group.Cycle stages are as follows: 1, diestrus; 2, proestrus; 3, estrus and 4, metestrus. Groups are as follows: (A) Control group; (B) PCOS group; (C) PCOS Lactobacillus transplantation group; (D) PCOS FMT group.

Mentions: Using daily vaginal smears, estrous cycles were analyzed for all rats. All control rats had regular estrous cycles of 4–5 days, comprising proestrus, estrus, metestrus and diestrus. The PCOS rats were constantly in diestrus stage, exhibiting predominantly leukocytes. On the 7th day after PCOS rats were treated with FMT, epithelial keratinocytes were observed microscopically during estrus in the vaginal smears of 3 rats; on the 14th day epithelial keratinocytes were observed in all of the 8 rats. The estrous cycle changes occurred twice in 5 rats, once in 3 rats. In the PCOS Lactobacillus transplantation group, 1 of 8 rats had epithelial keratinocytes on the 7th day; 6 of 8 rats had epithelial keratinocytes on the 14th day; the remaining 2 rats were in diestrus at the time of sacrifice. The estrous cycle changes occurred twice in 2 rats, once in 4 rats, and not at all in 2 rats (Fig 3).


Association between Polycystic Ovary Syndrome and Gut Microbiota.

Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, Tang L - PLoS ONE (2016)

Estrous cycle changes in three representative rats from each group.Cycle stages are as follows: 1, diestrus; 2, proestrus; 3, estrus and 4, metestrus. Groups are as follows: (A) Control group; (B) PCOS group; (C) PCOS Lactobacillus transplantation group; (D) PCOS FMT group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836746&req=5

pone.0153196.g003: Estrous cycle changes in three representative rats from each group.Cycle stages are as follows: 1, diestrus; 2, proestrus; 3, estrus and 4, metestrus. Groups are as follows: (A) Control group; (B) PCOS group; (C) PCOS Lactobacillus transplantation group; (D) PCOS FMT group.
Mentions: Using daily vaginal smears, estrous cycles were analyzed for all rats. All control rats had regular estrous cycles of 4–5 days, comprising proestrus, estrus, metestrus and diestrus. The PCOS rats were constantly in diestrus stage, exhibiting predominantly leukocytes. On the 7th day after PCOS rats were treated with FMT, epithelial keratinocytes were observed microscopically during estrus in the vaginal smears of 3 rats; on the 14th day epithelial keratinocytes were observed in all of the 8 rats. The estrous cycle changes occurred twice in 5 rats, once in 3 rats. In the PCOS Lactobacillus transplantation group, 1 of 8 rats had epithelial keratinocytes on the 7th day; 6 of 8 rats had epithelial keratinocytes on the 14th day; the remaining 2 rats were in diestrus at the time of sacrifice. The estrous cycle changes occurred twice in 2 rats, once in 4 rats, and not at all in 2 rats (Fig 3).

Bottom Line: Their ovarian morphologies normalized.The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella.These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.

ABSTRACT
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.

No MeSH data available.


Related in: MedlinePlus