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Diagnostic Value of Serum miR-182, miR-183, miR-210, and miR-126 Levels in Patients with Early-Stage Non-Small Cell Lung Cancer.

Zhu W, Zhou K, Zha Y, Chen D, He J, Ma H, Liu X, Le H, Zhang Y - PLoS ONE (2016)

Bottom Line: Blood-circulating miRNAs could be useful as a biomarker to detect lung cancer early.The data showed that the serum levels of miR-182, miR-183, and miR-210 were significantly upregulated and that the miR-126 level was significantly downregulated in NSCLC patients, compared with the healthy controls.Data from the current study validated that the four serum miRNAs could serve as a tumor biomarker for NSCLC early diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cytobiology and Molecular Biology, Zhoushan Hospital of Wenzhou Medical University, Zhoushan, Zhejiang, 316021, China.

ABSTRACT
Blood-circulating miRNAs could be useful as a biomarker to detect lung cancer early. We investigated the serum levels of four different miRNAs in patients with non-small cell lung cancer (NSCLC) and assessed their diagnostic value for NSCLC. Serum samples from 112 NSCLC patients and 104 controls (20 current smokers without lung cancer, 23 pneumonia patients, 21 gastric cancer patients, and 40 healthy controls) were subjected to Taqman probe-based quantitative reverse transcription-polymerase chain reaction (RT-PCR). The data showed that the serum levels of miR-182, miR-183, and miR-210 were significantly upregulated and that the miR-126 level was significantly downregulated in NSCLC patients, compared with the healthy controls. Further receiver operating characteristic (ROC) curve analysis revealed that the serum miR-182, miR-183, miR-210, or miR-126 level could serve as a diagnostic biomarker for NSCLC early detection, with a high sensitivity and specificity. The combination of these four miRNAs with carcinoembryonic antigen (CEA) further increased the diagnostic value, with an area under the curve (AUC) of 0.965 (sensitivity, 81.3%; specificity, 100.0%; and accuracy, 90.8%) using logistic regression model analysis. In addition, the relative levels of serum miR-182, miR-183, miR-210, and miR-126 could distinguish NSCLC or early-stage NSCLC from current tobacco smokers without lung cancer and pneumonia or gastric cancer patients with a high sensitivity and specificity. Data from the current study validated that the four serum miRNAs could serve as a tumor biomarker for NSCLC early diagnosis.

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Related in: MedlinePlus

Expression of miR-182, miR-183, miR-210, and miR-126 in sera from NSCLC patients and healthy controls.Graphs show dot-plots of medians and inter-quartile ranges of log2-transformed values of each miRNA in sera from 112 NSCLC patients (black) and 40 healthy controls (grey). U6 snRNA was used as the reference. P values of miR-182, miR-183, miR-210, and miR-126 were < 0.0001, 0.0181, 0.0299, and < 0.0001, respectively, using the Mann-Whitney test. *P < 0.05 between patients and controls.
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pone.0153046.g001: Expression of miR-182, miR-183, miR-210, and miR-126 in sera from NSCLC patients and healthy controls.Graphs show dot-plots of medians and inter-quartile ranges of log2-transformed values of each miRNA in sera from 112 NSCLC patients (black) and 40 healthy controls (grey). U6 snRNA was used as the reference. P values of miR-182, miR-183, miR-210, and miR-126 were < 0.0001, 0.0181, 0.0299, and < 0.0001, respectively, using the Mann-Whitney test. *P < 0.05 between patients and controls.

Mentions: Based on previous studies [9, 13–15, 22], we selected and assessed four dysregulated serum miRNAs in 216 samples (i.e., 112 NSCLC patients, 23 pneumonia patients, 21 gastric cancer patients, 20 current smokers without lung cancer, and 40 healthy controls). Our data showed that the levels of miR-182, miR-183, and miR-210 were significantly upregulated in the sera from NSCLC patients, compared to those of age- and gender-matched healthy controls (P < 0.0001, 0.0181, and 0.0299, respectively), whereas the serum miR-126 levels were significantly decreased in the NSCLC patients (P < 0.0001; Fig 1).


Diagnostic Value of Serum miR-182, miR-183, miR-210, and miR-126 Levels in Patients with Early-Stage Non-Small Cell Lung Cancer.

Zhu W, Zhou K, Zha Y, Chen D, He J, Ma H, Liu X, Le H, Zhang Y - PLoS ONE (2016)

Expression of miR-182, miR-183, miR-210, and miR-126 in sera from NSCLC patients and healthy controls.Graphs show dot-plots of medians and inter-quartile ranges of log2-transformed values of each miRNA in sera from 112 NSCLC patients (black) and 40 healthy controls (grey). U6 snRNA was used as the reference. P values of miR-182, miR-183, miR-210, and miR-126 were < 0.0001, 0.0181, 0.0299, and < 0.0001, respectively, using the Mann-Whitney test. *P < 0.05 between patients and controls.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836744&req=5

pone.0153046.g001: Expression of miR-182, miR-183, miR-210, and miR-126 in sera from NSCLC patients and healthy controls.Graphs show dot-plots of medians and inter-quartile ranges of log2-transformed values of each miRNA in sera from 112 NSCLC patients (black) and 40 healthy controls (grey). U6 snRNA was used as the reference. P values of miR-182, miR-183, miR-210, and miR-126 were < 0.0001, 0.0181, 0.0299, and < 0.0001, respectively, using the Mann-Whitney test. *P < 0.05 between patients and controls.
Mentions: Based on previous studies [9, 13–15, 22], we selected and assessed four dysregulated serum miRNAs in 216 samples (i.e., 112 NSCLC patients, 23 pneumonia patients, 21 gastric cancer patients, 20 current smokers without lung cancer, and 40 healthy controls). Our data showed that the levels of miR-182, miR-183, and miR-210 were significantly upregulated in the sera from NSCLC patients, compared to those of age- and gender-matched healthy controls (P < 0.0001, 0.0181, and 0.0299, respectively), whereas the serum miR-126 levels were significantly decreased in the NSCLC patients (P < 0.0001; Fig 1).

Bottom Line: Blood-circulating miRNAs could be useful as a biomarker to detect lung cancer early.The data showed that the serum levels of miR-182, miR-183, and miR-210 were significantly upregulated and that the miR-126 level was significantly downregulated in NSCLC patients, compared with the healthy controls.Data from the current study validated that the four serum miRNAs could serve as a tumor biomarker for NSCLC early diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cytobiology and Molecular Biology, Zhoushan Hospital of Wenzhou Medical University, Zhoushan, Zhejiang, 316021, China.

ABSTRACT
Blood-circulating miRNAs could be useful as a biomarker to detect lung cancer early. We investigated the serum levels of four different miRNAs in patients with non-small cell lung cancer (NSCLC) and assessed their diagnostic value for NSCLC. Serum samples from 112 NSCLC patients and 104 controls (20 current smokers without lung cancer, 23 pneumonia patients, 21 gastric cancer patients, and 40 healthy controls) were subjected to Taqman probe-based quantitative reverse transcription-polymerase chain reaction (RT-PCR). The data showed that the serum levels of miR-182, miR-183, and miR-210 were significantly upregulated and that the miR-126 level was significantly downregulated in NSCLC patients, compared with the healthy controls. Further receiver operating characteristic (ROC) curve analysis revealed that the serum miR-182, miR-183, miR-210, or miR-126 level could serve as a diagnostic biomarker for NSCLC early detection, with a high sensitivity and specificity. The combination of these four miRNAs with carcinoembryonic antigen (CEA) further increased the diagnostic value, with an area under the curve (AUC) of 0.965 (sensitivity, 81.3%; specificity, 100.0%; and accuracy, 90.8%) using logistic regression model analysis. In addition, the relative levels of serum miR-182, miR-183, miR-210, and miR-126 could distinguish NSCLC or early-stage NSCLC from current tobacco smokers without lung cancer and pneumonia or gastric cancer patients with a high sensitivity and specificity. Data from the current study validated that the four serum miRNAs could serve as a tumor biomarker for NSCLC early diagnosis.

Show MeSH
Related in: MedlinePlus