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Genomic Characteristics of Chinese Borrelia burgdorferi Isolates.

Hao Q, Du P, Zhang W, Hou X, Zhang L, Zhang Y, Liu H, Liu W, Chen C, Wan K - PLoS ONE (2016)

Bottom Line: The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies.Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31.The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

ABSTRACT
In China, B. burgdorferi, B.garinii, B. afzelii and B. yangtze sp. nov have been reported; B.garinii and B. afzelii are the main pathogenic genotypes. But until now only one Chinese strain was reported with whole genome sequence. In order to further understand the genomic characteristics and diversity of Chinese Borrelia strains, 5 isolates from China were sequenced and compared with the whole genome sequences of strains in other areas. The results showed a high degree of conservation within the linear chromosome of Chinese strains, whereas plasmid showed a much larger diversity according to the majority genomic information of plasmids. The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies. Pairwise analysis demonstrates that there are only 70 SNPs between the genomes of CS4 and B31. However, there are many more SNPs between the genomes of QX-S13 and VS116, PD91 and PBi, FP1 and PKo, R9 and Pko, respectively. Gene comparison showed some important different genes. OspA was one of the important different genes. Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31. OspA gene sequence of R9 had a 96bp deletion; OspA gene of PD91 had two deletions: 9bp and 10 bp. To conclude, we showed the genomic characteristics of four genotype Chinese B. burgdorferi strains. The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported. Comparative analysis of Chinese strains with the different Borrelia species from other areas will help us to understand evolution and pathogenesis of Chinese Borrelia burgdorferi strains.

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Whole genome phylogenetic analysis of 5 chinese strains and 26 reference strains.
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pone.0153149.g002: Whole genome phylogenetic analysis of 5 chinese strains and 26 reference strains.

Mentions: The result of phylogenetic analysis of 5 Chinese strains and 26 reference strains based on the whole genome showed all Borrelia burgdorferi strains had a same ancestor. The phylogenetic tree identified two pairs of statistically supported sister-group genomes from China: FP1-R9 and PD91-NMJW1(Fig 2).


Genomic Characteristics of Chinese Borrelia burgdorferi Isolates.

Hao Q, Du P, Zhang W, Hou X, Zhang L, Zhang Y, Liu H, Liu W, Chen C, Wan K - PLoS ONE (2016)

Whole genome phylogenetic analysis of 5 chinese strains and 26 reference strains.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836705&req=5

pone.0153149.g002: Whole genome phylogenetic analysis of 5 chinese strains and 26 reference strains.
Mentions: The result of phylogenetic analysis of 5 Chinese strains and 26 reference strains based on the whole genome showed all Borrelia burgdorferi strains had a same ancestor. The phylogenetic tree identified two pairs of statistically supported sister-group genomes from China: FP1-R9 and PD91-NMJW1(Fig 2).

Bottom Line: The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies.Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31.The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

ABSTRACT
In China, B. burgdorferi, B.garinii, B. afzelii and B. yangtze sp. nov have been reported; B.garinii and B. afzelii are the main pathogenic genotypes. But until now only one Chinese strain was reported with whole genome sequence. In order to further understand the genomic characteristics and diversity of Chinese Borrelia strains, 5 isolates from China were sequenced and compared with the whole genome sequences of strains in other areas. The results showed a high degree of conservation within the linear chromosome of Chinese strains, whereas plasmid showed a much larger diversity according to the majority genomic information of plasmids. The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies. Pairwise analysis demonstrates that there are only 70 SNPs between the genomes of CS4 and B31. However, there are many more SNPs between the genomes of QX-S13 and VS116, PD91 and PBi, FP1 and PKo, R9 and Pko, respectively. Gene comparison showed some important different genes. OspA was one of the important different genes. Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31. OspA gene sequence of R9 had a 96bp deletion; OspA gene of PD91 had two deletions: 9bp and 10 bp. To conclude, we showed the genomic characteristics of four genotype Chinese B. burgdorferi strains. The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported. Comparative analysis of Chinese strains with the different Borrelia species from other areas will help us to understand evolution and pathogenesis of Chinese Borrelia burgdorferi strains.

Show MeSH
Related in: MedlinePlus