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Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus

Protein alignment of putative Em-TSP3 isoforms with four transmembrane.Fully conserved residues are marked with (*), those replaced with amino acids of strongly similar properties with (:) and of weakly similar properties with (.) LEL variable region are in the solid line box and predicted transmembrane region are in the dashed line box. Protein mutant of the forth transmembrane is shaded in grey. There are three Em-TSP3 isoforms predicted, two of them are conserved with previous study [23], but one of them are intermediate type of the former two isoforms and need further verification.
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pntd.0004634.g006: Protein alignment of putative Em-TSP3 isoforms with four transmembrane.Fully conserved residues are marked with (*), those replaced with amino acids of strongly similar properties with (:) and of weakly similar properties with (.) LEL variable region are in the solid line box and predicted transmembrane region are in the dashed line box. Protein mutant of the forth transmembrane is shaded in grey. There are three Em-TSP3 isoforms predicted, two of them are conserved with previous study [23], but one of them are intermediate type of the former two isoforms and need further verification.

Mentions: Tetraspanins (TSPs) are a superfamily of plasma membrane-associated proteins consisting of four conserved transmembranes [58]. They have been used as vaccine candidates against schistosomiasis, echinococcosis and as diagnostic antigens for cysticercosis [2, 20, 59, 60]; In addition, it was proven that tetraspanins in the tegument of schistosomula and adult worms can act as receptors for host ligands, including MHC molecules, allowing parasites to mask their non-self-status and escape host immune responses [61]. A total of 11 amino acid sequences (Table 3) showed 91%-100% identity to the seven published Em-TSPs [20]. In addition, there were two putative Em-TSP3 isoforms and two amino acid sequences of one isoform and three amino sequences of another isoform (Table 3), and most mutation sites were located at the LEL variable region (Fig 6).


Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Protein alignment of putative Em-TSP3 isoforms with four transmembrane.Fully conserved residues are marked with (*), those replaced with amino acids of strongly similar properties with (:) and of weakly similar properties with (.) LEL variable region are in the solid line box and predicted transmembrane region are in the dashed line box. Protein mutant of the forth transmembrane is shaded in grey. There are three Em-TSP3 isoforms predicted, two of them are conserved with previous study [23], but one of them are intermediate type of the former two isoforms and need further verification.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836691&req=5

pntd.0004634.g006: Protein alignment of putative Em-TSP3 isoforms with four transmembrane.Fully conserved residues are marked with (*), those replaced with amino acids of strongly similar properties with (:) and of weakly similar properties with (.) LEL variable region are in the solid line box and predicted transmembrane region are in the dashed line box. Protein mutant of the forth transmembrane is shaded in grey. There are three Em-TSP3 isoforms predicted, two of them are conserved with previous study [23], but one of them are intermediate type of the former two isoforms and need further verification.
Mentions: Tetraspanins (TSPs) are a superfamily of plasma membrane-associated proteins consisting of four conserved transmembranes [58]. They have been used as vaccine candidates against schistosomiasis, echinococcosis and as diagnostic antigens for cysticercosis [2, 20, 59, 60]; In addition, it was proven that tetraspanins in the tegument of schistosomula and adult worms can act as receptors for host ligands, including MHC molecules, allowing parasites to mask their non-self-status and escape host immune responses [61]. A total of 11 amino acid sequences (Table 3) showed 91%-100% identity to the seven published Em-TSPs [20]. In addition, there were two putative Em-TSP3 isoforms and two amino acid sequences of one isoform and three amino sequences of another isoform (Table 3), and most mutation sites were located at the LEL variable region (Fig 6).

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus