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Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analyses of diagnostic antigen gp50 amino acid sequences.The neighbor-joining tree was constructed by MEGA 6.0 (www.megasoftware.net); bootstrap values were obtained from 1,000 replicates. Nonc: RPKM>100 at stages of non-activated oncosphere; Aonc: RPKM>100 at stages of activated oncosphere; P_gravid: FPKM>100 at stage of pre-gravid [23]; Gravid: FPKM>100 at stage of gravid [23].
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pntd.0004634.g005: Phylogenetic analyses of diagnostic antigen gp50 amino acid sequences.The neighbor-joining tree was constructed by MEGA 6.0 (www.megasoftware.net); bootstrap values were obtained from 1,000 replicates. Nonc: RPKM>100 at stages of non-activated oncosphere; Aonc: RPKM>100 at stages of activated oncosphere; P_gravid: FPKM>100 at stage of pre-gravid [23]; Gravid: FPKM>100 at stage of gravid [23].

Mentions: Taenia solium GP50 has been used for the diagnosis of cysticercosis [34]. GP50 isoforms are species-specific antigens and may be stage-specific in Cysticercus cellulosae [35] based on the lack of antibody reactivity with one serum sample from an individual confirmed to be taeniasis-positive but cysticercosis-negative [35]. A previous study showed that more than 90% of E. multilocularis GP50 isoforms were not expressed in metacestodes cultivated in vitro [23], and our present work also corroborated this finding, as few or no transcripts of GP50 were found in Cmet. Some GP50 isoforms were expressed in 4Wmet from in vivo DBA/2 mice infections, suggesting that these GP50 isoforms are key factors in the host-parasite interface during the early stage of infection. GP50 antigen family expression also showed quite high variability (Fig 5), and the lack of uniformity of isoform expression in oncospheres (non-activated and activated) and adults (pre-gravid and gravid) (Fig 5) indicates that the E. multilocularis diagnostic antigen GP50 may be stage-specific as well.


Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Phylogenetic analyses of diagnostic antigen gp50 amino acid sequences.The neighbor-joining tree was constructed by MEGA 6.0 (www.megasoftware.net); bootstrap values were obtained from 1,000 replicates. Nonc: RPKM>100 at stages of non-activated oncosphere; Aonc: RPKM>100 at stages of activated oncosphere; P_gravid: FPKM>100 at stage of pre-gravid [23]; Gravid: FPKM>100 at stage of gravid [23].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836691&req=5

pntd.0004634.g005: Phylogenetic analyses of diagnostic antigen gp50 amino acid sequences.The neighbor-joining tree was constructed by MEGA 6.0 (www.megasoftware.net); bootstrap values were obtained from 1,000 replicates. Nonc: RPKM>100 at stages of non-activated oncosphere; Aonc: RPKM>100 at stages of activated oncosphere; P_gravid: FPKM>100 at stage of pre-gravid [23]; Gravid: FPKM>100 at stage of gravid [23].
Mentions: Taenia solium GP50 has been used for the diagnosis of cysticercosis [34]. GP50 isoforms are species-specific antigens and may be stage-specific in Cysticercus cellulosae [35] based on the lack of antibody reactivity with one serum sample from an individual confirmed to be taeniasis-positive but cysticercosis-negative [35]. A previous study showed that more than 90% of E. multilocularis GP50 isoforms were not expressed in metacestodes cultivated in vitro [23], and our present work also corroborated this finding, as few or no transcripts of GP50 were found in Cmet. Some GP50 isoforms were expressed in 4Wmet from in vivo DBA/2 mice infections, suggesting that these GP50 isoforms are key factors in the host-parasite interface during the early stage of infection. GP50 antigen family expression also showed quite high variability (Fig 5), and the lack of uniformity of isoform expression in oncospheres (non-activated and activated) and adults (pre-gravid and gravid) (Fig 5) indicates that the E. multilocularis diagnostic antigen GP50 may be stage-specific as well.

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus