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Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus

Heatmap showing the Euclidean distances between the samples as calculated from the variance stabilizing transformation of the count data by DEGseq pakage [33].Nonc1: Non-activated oncosphere 1; Nonc2: Non-activated oncosphere2; Aonc: Activated oncosphere; 4Wmet: 4-week metacestodes miniature vesicles; Cmet: Metacestodes small vesicles cultivated in vitro.
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pntd.0004634.g003: Heatmap showing the Euclidean distances between the samples as calculated from the variance stabilizing transformation of the count data by DEGseq pakage [33].Nonc1: Non-activated oncosphere 1; Nonc2: Non-activated oncosphere2; Aonc: Activated oncosphere; 4Wmet: 4-week metacestodes miniature vesicles; Cmet: Metacestodes small vesicles cultivated in vitro.

Mentions: Larval tissue in the liver of 1–3 weeks post oral infections in DBA/2 mice were very small. After four weeks post oral infections, the lesions were identified in the livers and lesions with the parasite (4Wmet) were separated and extracted. The extracted sample contain more host tissue than the parasites which cause the number of reads were significantly decreased by filtering the mouse-mapped reads of 4Wmet (Table 2). But the cluster results showed a closer relationship with Cmet (Fig 3), which is in accordance with the biological development of E. multilocularis. For differentially expressed gene (DEG) analysis, we divided the cDNA libraries into biological development stages of non-activated oncosphere (Nonc1 and Nonc2), activated oncosphere (Aonc) and early stage metacestode (4Wmet, Cmet). There were 187 DEGs in the activated oncosphere versus non-activated oncosphere, 443 in activated oncosphere versus early stage metacestode and 1,433 in non-activated oncosphere versus early stage metacestode (Fig 4 and S2 Table). In total, there were 1,491 DEGs, and most of the genes identified between non-activated oncosphere versus early stage metacestode were also identified between activated oncosphere versus early stage metacestode (S2 Table). Almost DEGs were up-regulated when non-activated oncospheres transformed to activated oncospheres (Fig 4).


Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Heatmap showing the Euclidean distances between the samples as calculated from the variance stabilizing transformation of the count data by DEGseq pakage [33].Nonc1: Non-activated oncosphere 1; Nonc2: Non-activated oncosphere2; Aonc: Activated oncosphere; 4Wmet: 4-week metacestodes miniature vesicles; Cmet: Metacestodes small vesicles cultivated in vitro.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836691&req=5

pntd.0004634.g003: Heatmap showing the Euclidean distances between the samples as calculated from the variance stabilizing transformation of the count data by DEGseq pakage [33].Nonc1: Non-activated oncosphere 1; Nonc2: Non-activated oncosphere2; Aonc: Activated oncosphere; 4Wmet: 4-week metacestodes miniature vesicles; Cmet: Metacestodes small vesicles cultivated in vitro.
Mentions: Larval tissue in the liver of 1–3 weeks post oral infections in DBA/2 mice were very small. After four weeks post oral infections, the lesions were identified in the livers and lesions with the parasite (4Wmet) were separated and extracted. The extracted sample contain more host tissue than the parasites which cause the number of reads were significantly decreased by filtering the mouse-mapped reads of 4Wmet (Table 2). But the cluster results showed a closer relationship with Cmet (Fig 3), which is in accordance with the biological development of E. multilocularis. For differentially expressed gene (DEG) analysis, we divided the cDNA libraries into biological development stages of non-activated oncosphere (Nonc1 and Nonc2), activated oncosphere (Aonc) and early stage metacestode (4Wmet, Cmet). There were 187 DEGs in the activated oncosphere versus non-activated oncosphere, 443 in activated oncosphere versus early stage metacestode and 1,433 in non-activated oncosphere versus early stage metacestode (Fig 4 and S2 Table). In total, there were 1,491 DEGs, and most of the genes identified between non-activated oncosphere versus early stage metacestode were also identified between activated oncosphere versus early stage metacestode (S2 Table). Almost DEGs were up-regulated when non-activated oncospheres transformed to activated oncospheres (Fig 4).

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus