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Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus

Morphology of different life cycle stages of E. multilocularis.A: Egg; B: Oncospheres (non-activated); C: Oncospheres (activating) with the hooks dispersive and body swelling; D: Oncospheres (activated) with the hooks aggregation in the smaller lobe after 24 hours activation; E: 4-week metacestodes miniature vesicles; F: Metacestodes small vesicles cultivated in vitro; Bar: 10μm (A-E), 1mm (F); Arrowhead: Miniature vesicles.
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pntd.0004634.g001: Morphology of different life cycle stages of E. multilocularis.A: Egg; B: Oncospheres (non-activated); C: Oncospheres (activating) with the hooks dispersive and body swelling; D: Oncospheres (activated) with the hooks aggregation in the smaller lobe after 24 hours activation; E: 4-week metacestodes miniature vesicles; F: Metacestodes small vesicles cultivated in vitro; Bar: 10μm (A-E), 1mm (F); Arrowhead: Miniature vesicles.

Mentions: In vitro cultivation of E. multilocularis was carried out as described previously [25, 26]. In short, cyst masses of metacestodes from intraperitoneal passage DBA/2 mice at 16 weeks were cut into small pieces and cultivated in DMEM (Gibco) with 10% fetal calf serum (Gibco) at 37°C. Miniature cysts were grown to small vesicles (2–4 mm in diameter) in several weeks but were harvested before the formation of brood capsules and protoscoleces (Fig 1).


Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.

Huang F, Dang Z, Suzuki Y, Horiuchi T, Yagi K, Kouguchi H, Irie T, Kim K, Oku Y - PLoS Negl Trop Dis (2016)

Morphology of different life cycle stages of E. multilocularis.A: Egg; B: Oncospheres (non-activated); C: Oncospheres (activating) with the hooks dispersive and body swelling; D: Oncospheres (activated) with the hooks aggregation in the smaller lobe after 24 hours activation; E: 4-week metacestodes miniature vesicles; F: Metacestodes small vesicles cultivated in vitro; Bar: 10μm (A-E), 1mm (F); Arrowhead: Miniature vesicles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836691&req=5

pntd.0004634.g001: Morphology of different life cycle stages of E. multilocularis.A: Egg; B: Oncospheres (non-activated); C: Oncospheres (activating) with the hooks dispersive and body swelling; D: Oncospheres (activated) with the hooks aggregation in the smaller lobe after 24 hours activation; E: 4-week metacestodes miniature vesicles; F: Metacestodes small vesicles cultivated in vitro; Bar: 10μm (A-E), 1mm (F); Arrowhead: Miniature vesicles.
Mentions: In vitro cultivation of E. multilocularis was carried out as described previously [25, 26]. In short, cyst masses of metacestodes from intraperitoneal passage DBA/2 mice at 16 weeks were cut into small pieces and cultivated in DMEM (Gibco) with 10% fetal calf serum (Gibco) at 37°C. Miniature cysts were grown to small vesicles (2–4 mm in diameter) in several weeks but were harvested before the formation of brood capsules and protoscoleces (Fig 1).

Bottom Line: However, little gene expression data is available for antigens of the egg and early larval stages.Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages.The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.

ABSTRACT
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

No MeSH data available.


Related in: MedlinePlus