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A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


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Phenotypes of genetic complementation in transgenic S1-24 mutant plants.(A) Gross morphologies of wild-type, S1-24, and R1 and R2 complementation lines at the mature stage. (B) Resistance to breakage in culms of the R1 and R2 complementation lines compared with S1-24 and wild-type culms.
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pone.0153993.g006: Phenotypes of genetic complementation in transgenic S1-24 mutant plants.(A) Gross morphologies of wild-type, S1-24, and R1 and R2 complementation lines at the mature stage. (B) Resistance to breakage in culms of the R1 and R2 complementation lines compared with S1-24 and wild-type culms.

Mentions: The identity of OsCESA7 was confirmed by genetic complementation analysis. A plasmid containing the entire OsCESA7 coding region and promoter was introduced into the S1-24 mutant. Approximately 10 lines and 100 independent transgenic plants were obtained. Eight lines, including R1 and R2, showed restoration of the wild-type phenotype in the S1-24 mutant background. The R1 and R2 lines exhibited phenotypes similar to the wild type, the most obvious being the absence of brittle culms (Fig 6). Therefore, we conclude that the mutation in OsCESA7 leads to the brittle culm phenotype.


A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Phenotypes of genetic complementation in transgenic S1-24 mutant plants.(A) Gross morphologies of wild-type, S1-24, and R1 and R2 complementation lines at the mature stage. (B) Resistance to breakage in culms of the R1 and R2 complementation lines compared with S1-24 and wild-type culms.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g006: Phenotypes of genetic complementation in transgenic S1-24 mutant plants.(A) Gross morphologies of wild-type, S1-24, and R1 and R2 complementation lines at the mature stage. (B) Resistance to breakage in culms of the R1 and R2 complementation lines compared with S1-24 and wild-type culms.
Mentions: The identity of OsCESA7 was confirmed by genetic complementation analysis. A plasmid containing the entire OsCESA7 coding region and promoter was introduced into the S1-24 mutant. Approximately 10 lines and 100 independent transgenic plants were obtained. Eight lines, including R1 and R2, showed restoration of the wild-type phenotype in the S1-24 mutant background. The R1 and R2 lines exhibited phenotypes similar to the wild type, the most obvious being the absence of brittle culms (Fig 6). Therefore, we conclude that the mutation in OsCESA7 leads to the brittle culm phenotype.

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus