Limits...
A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus

Polysaccharide content of cell walls in the second upper internodes at the heading stage.Values shown are averages of values for five plants. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g004: Polysaccharide content of cell walls in the second upper internodes at the heading stage.Values shown are averages of values for five plants. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.

Mentions: The defect in cell wall thickening suggests that the cell wall composition might also be altered. To address this possibility, four polysaccharide fractions (hot water, pectin, hemicellulose, and cellulose), which are the main components of cell walls, were extracted from culms, and the proportions of each fraction were quantified. The amount of cellulose in S1-24 mutant culms was reduced to 48% that of wild-type culms (Fig 4). In contrast, the amount of hemicellulose in the S1-24 culms was 27% higher than in the wild type. The main function of cellulose is mechanical support, so the low mechanical strength in the S1-24 mutant was caused primarily by decreased cellulose content. The increase in hemicellulose in the S1-24 mutant may be the result of a compensation reaction. The mutated gene in the S1-24 mutant appeared to play an important role in cellulose biosynthesis and to affect cell wall morphogenesis.


A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Polysaccharide content of cell walls in the second upper internodes at the heading stage.Values shown are averages of values for five plants. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g004: Polysaccharide content of cell walls in the second upper internodes at the heading stage.Values shown are averages of values for five plants. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
Mentions: The defect in cell wall thickening suggests that the cell wall composition might also be altered. To address this possibility, four polysaccharide fractions (hot water, pectin, hemicellulose, and cellulose), which are the main components of cell walls, were extracted from culms, and the proportions of each fraction were quantified. The amount of cellulose in S1-24 mutant culms was reduced to 48% that of wild-type culms (Fig 4). In contrast, the amount of hemicellulose in the S1-24 culms was 27% higher than in the wild type. The main function of cellulose is mechanical support, so the low mechanical strength in the S1-24 mutant was caused primarily by decreased cellulose content. The increase in hemicellulose in the S1-24 mutant may be the result of a compensation reaction. The mutated gene in the S1-24 mutant appeared to play an important role in cellulose biosynthesis and to affect cell wall morphogenesis.

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus