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A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus

Cross section of a culm viewed under a scanning electron microscope.(A, B) Cross section of a wild-type culm. (C, D) Cross section of an S1-24 culm. Magnification in the images is 500× (A, C) or 5000 × (B, D). Green and yellow arrows represented thickened sclerenchyma cell (SC) walls and unthicken parenchyma cell (PC) walls.
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pone.0153993.g003: Cross section of a culm viewed under a scanning electron microscope.(A, B) Cross section of a wild-type culm. (C, D) Cross section of an S1-24 culm. Magnification in the images is 500× (A, C) or 5000 × (B, D). Green and yellow arrows represented thickened sclerenchyma cell (SC) walls and unthicken parenchyma cell (PC) walls.

Mentions: To determine the cause of the reduction in mechanical strength in the S1-24 mutant, culm cross sections were examined under a scanning electron microscope. As shown in Fig 3, obvious differences were found between the mutant and wild-type culms. In the wild-type culms, the sclerenchyma cell walls, which provide the main structural support for the plant body, were obviously thick (Fig 3A and 3B). In contrast, the sclerenchyma cell walls in the mutant showed no significant thickening (Fig 3C and 3D). No obvious differences between parenchyma cells in wild-type and mutant culms were observed (Fig 3A and 3C). Consequently, the reduction in mechanical strength in the S1-24 mutant was due to a defect in thickening of the sclerenchyma cell wall.


A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Cross section of a culm viewed under a scanning electron microscope.(A, B) Cross section of a wild-type culm. (C, D) Cross section of an S1-24 culm. Magnification in the images is 500× (A, C) or 5000 × (B, D). Green and yellow arrows represented thickened sclerenchyma cell (SC) walls and unthicken parenchyma cell (PC) walls.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g003: Cross section of a culm viewed under a scanning electron microscope.(A, B) Cross section of a wild-type culm. (C, D) Cross section of an S1-24 culm. Magnification in the images is 500× (A, C) or 5000 × (B, D). Green and yellow arrows represented thickened sclerenchyma cell (SC) walls and unthicken parenchyma cell (PC) walls.
Mentions: To determine the cause of the reduction in mechanical strength in the S1-24 mutant, culm cross sections were examined under a scanning electron microscope. As shown in Fig 3, obvious differences were found between the mutant and wild-type culms. In the wild-type culms, the sclerenchyma cell walls, which provide the main structural support for the plant body, were obviously thick (Fig 3A and 3B). In contrast, the sclerenchyma cell walls in the mutant showed no significant thickening (Fig 3C and 3D). No obvious differences between parenchyma cells in wild-type and mutant culms were observed (Fig 3A and 3C). Consequently, the reduction in mechanical strength in the S1-24 mutant was due to a defect in thickening of the sclerenchyma cell wall.

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus