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A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus

Pleiotropic phenotypes of the S1-24 mutant.(A) Gross morphology at the seedling stage (bar = 2 cm). (B) Withering in the flag leaf apex of the S1-24 mutant. (C) Gross morphology at the mature stage (bar = 12 cm). (D) Plant height at the mature stage. (E) Tiller number at the mature stage. (F) Seed setting rate. Values shown are the averages of values for 10 plants. Bars represent standard errors. ** indicates statistical significance by a t test at P < 0.01.
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pone.0153993.g002: Pleiotropic phenotypes of the S1-24 mutant.(A) Gross morphology at the seedling stage (bar = 2 cm). (B) Withering in the flag leaf apex of the S1-24 mutant. (C) Gross morphology at the mature stage (bar = 12 cm). (D) Plant height at the mature stage. (E) Tiller number at the mature stage. (F) Seed setting rate. Values shown are the averages of values for 10 plants. Bars represent standard errors. ** indicates statistical significance by a t test at P < 0.01.

Mentions: In addition to reduced mechanical strength, the S1-24 mutant exhibited other pleiotropic phenotypes. The mutant showed slight dwarfism with withered leaf tips at the seedling stage (Fig 2A and 2B). These phenotypes became more obvious at the mature stage. Leaves and stems with considerable drooping were observed (Fig 2C), and plant height of the S1-24 mutant was reduced to only 50% of that of wild-type plants (Fig 2D). In addition, the tiller number of the mutant was decreased by 33%, and the rate of seed setting decreased by approximately 23% (Fig 2E and 2F).


A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Pleiotropic phenotypes of the S1-24 mutant.(A) Gross morphology at the seedling stage (bar = 2 cm). (B) Withering in the flag leaf apex of the S1-24 mutant. (C) Gross morphology at the mature stage (bar = 12 cm). (D) Plant height at the mature stage. (E) Tiller number at the mature stage. (F) Seed setting rate. Values shown are the averages of values for 10 plants. Bars represent standard errors. ** indicates statistical significance by a t test at P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g002: Pleiotropic phenotypes of the S1-24 mutant.(A) Gross morphology at the seedling stage (bar = 2 cm). (B) Withering in the flag leaf apex of the S1-24 mutant. (C) Gross morphology at the mature stage (bar = 12 cm). (D) Plant height at the mature stage. (E) Tiller number at the mature stage. (F) Seed setting rate. Values shown are the averages of values for 10 plants. Bars represent standard errors. ** indicates statistical significance by a t test at P < 0.01.
Mentions: In addition to reduced mechanical strength, the S1-24 mutant exhibited other pleiotropic phenotypes. The mutant showed slight dwarfism with withered leaf tips at the seedling stage (Fig 2A and 2B). These phenotypes became more obvious at the mature stage. Leaves and stems with considerable drooping were observed (Fig 2C), and plant height of the S1-24 mutant was reduced to only 50% of that of wild-type plants (Fig 2D). In addition, the tiller number of the mutant was decreased by 33%, and the rate of seed setting decreased by approximately 23% (Fig 2E and 2F).

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus