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A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus

Phenotypes and physical properties of the S1-24 mutant.(A) An easily broken culm of S1-24 compared with the wild type. (B) An easily broken flag leaf of S1-24 compared with the wild type. (C, D) Force required to break the first and second upper internodes. (E, F) Elongation length of the first and second upper internodes. Values shown are the averages of values for five internodes. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
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pone.0153993.g001: Phenotypes and physical properties of the S1-24 mutant.(A) An easily broken culm of S1-24 compared with the wild type. (B) An easily broken flag leaf of S1-24 compared with the wild type. (C, D) Force required to break the first and second upper internodes. (E, F) Elongation length of the first and second upper internodes. Values shown are the averages of values for five internodes. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.

Mentions: The S1-24 mutant was isolated from plants of the japonica cultivar Nipponbare mutagenized with EMS; it is characterized by brittle culms and leaves that can be easily broken by bending (Fig 1A and 1B). Two parameters that are important for a precise description of this phenotype were measured: the breaking force and the elongation length, which are parameters that define the force required to break a culm segment and the elasticity of the plant tissue, respectively. We measured the mechanical strength of the first and second internodes of wild-type and mutant plants at the heading stage. The breaking force of the first and second upper internodes of the S1-24 mutant was reduced by 79% and 80%, respectively, compared with the wild-type plants (Fig 1C and 1D). As shown in Fig 1E and 1F, the elongation length of the first and second internodes of the mutant was reduced by 74% and 73%, respectively, compared with wild-type plants. These results showed that the mutation in S1-24 has a strong effect on the mechanical strength of rice plants.


A Missense Mutation in the Zinc Finger Domain of OsCESA7 Deleteriously Affects Cellulose Biosynthesis and Plant Growth in Rice.

Wang D, Qin Y, Fang J, Yuan S, Peng L, Zhao J, Li X - PLoS ONE (2016)

Phenotypes and physical properties of the S1-24 mutant.(A) An easily broken culm of S1-24 compared with the wild type. (B) An easily broken flag leaf of S1-24 compared with the wild type. (C, D) Force required to break the first and second upper internodes. (E, F) Elongation length of the first and second upper internodes. Values shown are the averages of values for five internodes. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836682&req=5

pone.0153993.g001: Phenotypes and physical properties of the S1-24 mutant.(A) An easily broken culm of S1-24 compared with the wild type. (B) An easily broken flag leaf of S1-24 compared with the wild type. (C, D) Force required to break the first and second upper internodes. (E, F) Elongation length of the first and second upper internodes. Values shown are the averages of values for five internodes. Bars represent standard errors. ** indicate statistical significance by a t test at P < 0.01.
Mentions: The S1-24 mutant was isolated from plants of the japonica cultivar Nipponbare mutagenized with EMS; it is characterized by brittle culms and leaves that can be easily broken by bending (Fig 1A and 1B). Two parameters that are important for a precise description of this phenotype were measured: the breaking force and the elongation length, which are parameters that define the force required to break a culm segment and the elasticity of the plant tissue, respectively. We measured the mechanical strength of the first and second internodes of wild-type and mutant plants at the heading stage. The breaking force of the first and second upper internodes of the S1-24 mutant was reduced by 79% and 80%, respectively, compared with the wild-type plants (Fig 1C and 1D). As shown in Fig 1E and 1F, the elongation length of the first and second internodes of the mutant was reduced by 74% and 73%, respectively, compared with wild-type plants. These results showed that the mutation in S1-24 has a strong effect on the mechanical strength of rice plants.

Bottom Line: The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant.The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm.These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Rice is a model plant species for the study of cellulose biosynthesis. We isolated a mutant, S1-24, from ethyl methanesulfonate (EMS)-treated plants of the japonica rice cultivar, Nipponbare. The mutant exhibited brittle culms and other pleiotropic phenotypes such as dwarfism and partial sterility. The brittle culms resulted from reduced mechanical strength due to a defect in thickening of the sclerenchyma cell wall and reduced cellulose content in the culms of the S1-24 mutant. Map-based gene cloning and a complementation assay showed that phenotypes of the S1-24 mutant were caused by a recessive point mutation in the OsCESA7 gene, which encodes cellulose synthase A subunit 7. The missense mutation changed the highly conserved C40 to Y in the zinc finger domain. The OsCESA7 gene is expressed predominantly in the culm at the mature stage, particularly in mechanical tissues such as vascular bundles and sclerenchyma cells, consistent with the brittle phenotype in the culm. These results indicate that OsCESA7 plays an important role in cellulose biosynthesis and plant growth.

No MeSH data available.


Related in: MedlinePlus