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Evolution of Pre- and Post-Copulatory Traits in Male Drosophila melanogaster as a Correlated Response to Selection for Resistance to Cold Stress.

Singh K, Samant MA, Tom MT, Prasad NG - PLoS ONE (2016)

Bottom Line: In Drosophila melanogaster the fitness of males depends on a broad array of reproductive traits classified as pre- and post-copulatory traits.Exposure to cold stress, can reduce sperm number, male mating ability and courtship behavior.Additionally, improved performances under stressful conditions need not necessarily trade-off with performance under benign conditions.

View Article: PubMed Central - PubMed

Affiliation: Indian Institute of Science Education and Research Mohali, Knowledge City, Sector 81, SAS Nagar, PO Manauli, Punjab, 140306, India.

ABSTRACT

Background: In Drosophila melanogaster the fitness of males depends on a broad array of reproductive traits classified as pre- and post-copulatory traits. Exposure to cold stress, can reduce sperm number, male mating ability and courtship behavior. Therefore, it is expected that the adaptation to cold stress will involve changes in pre- and post-copulatory traits. Such evolution of reproductive traits in response to cold stress is not well studied.

Methods: We selected replicate populations of D. melanogaster for resistance to cold shock. Over 37-46 generations of selection, we investigated pre- and post-copulatory traits such as mating latency, copulation duration, mating frequency, male fertility, fitness (progeny production) and sperm competitive ability in male flies subjected to cold shock and those not subjected to cold shock.

Results: We found that post cold shock, the males from the selected populations had a significantly lower mating latency along with, higher mating frequency, fertility, sperm competitive ability and number of progeny relative to the control populations.

Conclusion: While most studies of experimental evolution of cold stress resistance have documented the evolution of survivorship in response to selection, our study clearly shows that adaptation to cold stress involves rapid changes in the pre- and post-copulatory traits. Additionally, improved performances under stressful conditions need not necessarily trade-off with performance under benign conditions.

No MeSH data available.


Related in: MedlinePlus

Effect of cold shock (A) or no shock (B) on mating latency. (A) We assayed mating latency at 4 hours (h), 12 h and 30 h post cold shock. Closed bars represent FCB and open bars represent FSB populations. Selection and period had significant effects on mating latency. However, selection × period interaction was not significant. (B) Under no-shock treatment, there was no significant difference in mating latency between FSB and FCB males.
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pone.0153629.g001: Effect of cold shock (A) or no shock (B) on mating latency. (A) We assayed mating latency at 4 hours (h), 12 h and 30 h post cold shock. Closed bars represent FCB and open bars represent FSB populations. Selection and period had significant effects on mating latency. However, selection × period interaction was not significant. (B) Under no-shock treatment, there was no significant difference in mating latency between FSB and FCB males.

Mentions: Mating latency has evolved in response to selection. We found significant effects of the selection (FSB and FCB), period (4, 12 and 30 hours) and block (1–5) on mating latency (Table 1A, Fig 1A). FSB males on an average start mating 4 minutes earlier than FCB males after being subjected to cold shock (and combined with virgin base line females) (Fig 1A). Multiple comparisons employing Tukey’s HSD indicated that in both FSB and FCB populations, mating latency measured at 4 hours post cold shock was significantly higher relative to mating latency measured at 12 and 30 hours post cold shock. There was no significant selection × period interaction (Table 1A, Fig 1A). While block had a significant effect, none of the interactions involving block were significant (Table 1A).


Evolution of Pre- and Post-Copulatory Traits in Male Drosophila melanogaster as a Correlated Response to Selection for Resistance to Cold Stress.

Singh K, Samant MA, Tom MT, Prasad NG - PLoS ONE (2016)

Effect of cold shock (A) or no shock (B) on mating latency. (A) We assayed mating latency at 4 hours (h), 12 h and 30 h post cold shock. Closed bars represent FCB and open bars represent FSB populations. Selection and period had significant effects on mating latency. However, selection × period interaction was not significant. (B) Under no-shock treatment, there was no significant difference in mating latency between FSB and FCB males.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836659&req=5

pone.0153629.g001: Effect of cold shock (A) or no shock (B) on mating latency. (A) We assayed mating latency at 4 hours (h), 12 h and 30 h post cold shock. Closed bars represent FCB and open bars represent FSB populations. Selection and period had significant effects on mating latency. However, selection × period interaction was not significant. (B) Under no-shock treatment, there was no significant difference in mating latency between FSB and FCB males.
Mentions: Mating latency has evolved in response to selection. We found significant effects of the selection (FSB and FCB), period (4, 12 and 30 hours) and block (1–5) on mating latency (Table 1A, Fig 1A). FSB males on an average start mating 4 minutes earlier than FCB males after being subjected to cold shock (and combined with virgin base line females) (Fig 1A). Multiple comparisons employing Tukey’s HSD indicated that in both FSB and FCB populations, mating latency measured at 4 hours post cold shock was significantly higher relative to mating latency measured at 12 and 30 hours post cold shock. There was no significant selection × period interaction (Table 1A, Fig 1A). While block had a significant effect, none of the interactions involving block were significant (Table 1A).

Bottom Line: In Drosophila melanogaster the fitness of males depends on a broad array of reproductive traits classified as pre- and post-copulatory traits.Exposure to cold stress, can reduce sperm number, male mating ability and courtship behavior.Additionally, improved performances under stressful conditions need not necessarily trade-off with performance under benign conditions.

View Article: PubMed Central - PubMed

Affiliation: Indian Institute of Science Education and Research Mohali, Knowledge City, Sector 81, SAS Nagar, PO Manauli, Punjab, 140306, India.

ABSTRACT

Background: In Drosophila melanogaster the fitness of males depends on a broad array of reproductive traits classified as pre- and post-copulatory traits. Exposure to cold stress, can reduce sperm number, male mating ability and courtship behavior. Therefore, it is expected that the adaptation to cold stress will involve changes in pre- and post-copulatory traits. Such evolution of reproductive traits in response to cold stress is not well studied.

Methods: We selected replicate populations of D. melanogaster for resistance to cold shock. Over 37-46 generations of selection, we investigated pre- and post-copulatory traits such as mating latency, copulation duration, mating frequency, male fertility, fitness (progeny production) and sperm competitive ability in male flies subjected to cold shock and those not subjected to cold shock.

Results: We found that post cold shock, the males from the selected populations had a significantly lower mating latency along with, higher mating frequency, fertility, sperm competitive ability and number of progeny relative to the control populations.

Conclusion: While most studies of experimental evolution of cold stress resistance have documented the evolution of survivorship in response to selection, our study clearly shows that adaptation to cold stress involves rapid changes in the pre- and post-copulatory traits. Additionally, improved performances under stressful conditions need not necessarily trade-off with performance under benign conditions.

No MeSH data available.


Related in: MedlinePlus