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Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus

Larvae survival rates with spores and phage cocktail (PC) treatments. Larvae were fed spores, phage cocktail, spores and then phage cocktail 4 hours later, or phage cocktail and then spores 4 hours later. Sample sizes were as follows: 2188 (1 dose), n = 51 and 49; broth control, n = 48 and n = 48; phage cocktail control, n = 48 and 49; treatment (cocktail administered after infection with spores), n = 54 and 53; and prophylaxis treatment (cocktail administered prior to infection), n = 52 and 53. Error bars = standard error.
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f0005: Larvae survival rates with spores and phage cocktail (PC) treatments. Larvae were fed spores, phage cocktail, spores and then phage cocktail 4 hours later, or phage cocktail and then spores 4 hours later. Sample sizes were as follows: 2188 (1 dose), n = 51 and 49; broth control, n = 48 and n = 48; phage cocktail control, n = 48 and 49; treatment (cocktail administered after infection with spores), n = 54 and 53; and prophylaxis treatment (cocktail administered prior to infection), n = 52 and 53. Error bars = standard error.


Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Larvae survival rates with spores and phage cocktail (PC) treatments. Larvae were fed spores, phage cocktail, spores and then phage cocktail 4 hours later, or phage cocktail and then spores 4 hours later. Sample sizes were as follows: 2188 (1 dose), n = 51 and 49; broth control, n = 48 and n = 48; phage cocktail control, n = 48 and 49; treatment (cocktail administered after infection with spores), n = 54 and 53; and prophylaxis treatment (cocktail administered prior to infection), n = 52 and 53. Error bars = standard error.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836486&req=5

f0005: Larvae survival rates with spores and phage cocktail (PC) treatments. Larvae were fed spores, phage cocktail, spores and then phage cocktail 4 hours later, or phage cocktail and then spores 4 hours later. Sample sizes were as follows: 2188 (1 dose), n = 51 and 49; broth control, n = 48 and n = 48; phage cocktail control, n = 48 and 49; treatment (cocktail administered after infection with spores), n = 54 and 53; and prophylaxis treatment (cocktail administered prior to infection), n = 52 and 53. Error bars = standard error.
Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus