Limits...
Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus

Larvae survival rates of media controls. Survival of larvae treated with a negative control (food only) and with the addition of broth (200 µl per 1.0 ml food). Larvae were harvested on Day 0 and fed daily according to Table 3. Negative control data represents 10 control replicates with n = 12 or 13 each. Error bars = standard error.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836486&req=5

f0003: Larvae survival rates of media controls. Survival of larvae treated with a negative control (food only) and with the addition of broth (200 µl per 1.0 ml food). Larvae were harvested on Day 0 and fed daily according to Table 3. Negative control data represents 10 control replicates with n = 12 or 13 each. Error bars = standard error.

Mentions: The percent larval survival on the last day of pupation, Day 8, was compared using the student's t-test for p-value calculations. Significant differences were present in each of the following comparisons: negative controls versus ATTC 49843 spore infections (p = 3.99 × 10-8); negative controls versus NRRL B-3554 spore infections (p = 3.58 × 10-8); negative controls versus 2188 spore infections with one dose (p = 2.11 × 10-6); 2188 spore infection with one dose versus phage cocktail prophylaxis (p = 0.0193). All other comparisons yielded p-values that were not significantly different. Results showing comparisons of the actual survival rates between different groups during the experiments can be visualized in Figs. 3–5.Figure 3.


Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Larvae survival rates of media controls. Survival of larvae treated with a negative control (food only) and with the addition of broth (200 µl per 1.0 ml food). Larvae were harvested on Day 0 and fed daily according to Table 3. Negative control data represents 10 control replicates with n = 12 or 13 each. Error bars = standard error.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836486&req=5

f0003: Larvae survival rates of media controls. Survival of larvae treated with a negative control (food only) and with the addition of broth (200 µl per 1.0 ml food). Larvae were harvested on Day 0 and fed daily according to Table 3. Negative control data represents 10 control replicates with n = 12 or 13 each. Error bars = standard error.
Mentions: The percent larval survival on the last day of pupation, Day 8, was compared using the student's t-test for p-value calculations. Significant differences were present in each of the following comparisons: negative controls versus ATTC 49843 spore infections (p = 3.99 × 10-8); negative controls versus NRRL B-3554 spore infections (p = 3.58 × 10-8); negative controls versus 2188 spore infections with one dose (p = 2.11 × 10-6); 2188 spore infection with one dose versus phage cocktail prophylaxis (p = 0.0193). All other comparisons yielded p-values that were not significantly different. Results showing comparisons of the actual survival rates between different groups during the experiments can be visualized in Figs. 3–5.Figure 3.

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus