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Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus

Representative TEM images of 2 phage isolates. The left image displays a phage isolated from a propolis sample with a prolate head and the right image displays a phage isolated from a cosmetic source with an icosahedral head. Scale bar = 100 nm.
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f0002: Representative TEM images of 2 phage isolates. The left image displays a phage isolated from a propolis sample with a prolate head and the right image displays a phage isolated from a cosmetic source with an icosahedral head. Scale bar = 100 nm.

Mentions: Results for these 7 phages were imaged using TEM (Table 2). All seven phages had icosahedral or prolate heads with long, flexible tails, which places them in the family Siphoviridae. Even among phages in the same family, there are size variations of heads and tails.9 Images representing a prolate phage isolate and an icosahedral phage isolate are provided in Fig. 2.Figure 2.


Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Representative TEM images of 2 phage isolates. The left image displays a phage isolated from a propolis sample with a prolate head and the right image displays a phage isolated from a cosmetic source with an icosahedral head. Scale bar = 100 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836486&req=5

f0002: Representative TEM images of 2 phage isolates. The left image displays a phage isolated from a propolis sample with a prolate head and the right image displays a phage isolated from a cosmetic source with an icosahedral head. Scale bar = 100 nm.
Mentions: Results for these 7 phages were imaged using TEM (Table 2). All seven phages had icosahedral or prolate heads with long, flexible tails, which places them in the family Siphoviridae. Even among phages in the same family, there are size variations of heads and tails.9 Images representing a prolate phage isolate and an icosahedral phage isolate are provided in Fig. 2.Figure 2.

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus