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Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus

Host range of isolated P. larvae bacteriophages determined by soft agar overlay spot tests. Results range from no lysis (blank cell) to complete lysis (black cell). The bacterial species are represented across the top and are ranked from left to right in order of susceptibility to lysis. The isolated phages are listed on the left side of the table and are ranked from top to bottom in order of the percentage of P. larvae strains they are capable of lysing.
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f0001: Host range of isolated P. larvae bacteriophages determined by soft agar overlay spot tests. Results range from no lysis (blank cell) to complete lysis (black cell). The bacterial species are represented across the top and are ranked from left to right in order of susceptibility to lysis. The isolated phages are listed on the left side of the table and are ranked from top to bottom in order of the percentage of P. larvae strains they are capable of lysing.

Mentions: Specificity of the isolated phages on the genus Paenibacillus, and more precisely, to the species P. larvae, was determined by spot test experiments (Fig. 1). Bacterial strains were selected to test closely related organisms (such as P. alvei) and species not closely related (such as E. coli W3104). The phages showed high specificity to P. larvae strains with some isolates capable of a minor lysis of an unknown species of Paenibacillus isolated from an infected hive. This organism was not identified to the species level after 16sRNA gene sequencing failed to identify this species (data not shown).Figure 1.


Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.

Yost DG, Tsourkas P, Amy PS - Bacteriophage (2016)

Host range of isolated P. larvae bacteriophages determined by soft agar overlay spot tests. Results range from no lysis (blank cell) to complete lysis (black cell). The bacterial species are represented across the top and are ranked from left to right in order of susceptibility to lysis. The isolated phages are listed on the left side of the table and are ranked from top to bottom in order of the percentage of P. larvae strains they are capable of lysing.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836486&req=5

f0001: Host range of isolated P. larvae bacteriophages determined by soft agar overlay spot tests. Results range from no lysis (blank cell) to complete lysis (black cell). The bacterial species are represented across the top and are ranked from left to right in order of susceptibility to lysis. The isolated phages are listed on the left side of the table and are ranked from top to bottom in order of the percentage of P. larvae strains they are capable of lysing.
Mentions: Specificity of the isolated phages on the genus Paenibacillus, and more precisely, to the species P. larvae, was determined by spot test experiments (Fig. 1). Bacterial strains were selected to test closely related organisms (such as P. alvei) and species not closely related (such as E. coli W3104). The phages showed high specificity to P. larvae strains with some isolates capable of a minor lysis of an unknown species of Paenibacillus isolated from an infected hive. This organism was not identified to the species level after 16sRNA gene sequencing failed to identify this species (data not shown).Figure 1.

Bottom Line: Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae.Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted.Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

ABSTRACT

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

No MeSH data available.


Related in: MedlinePlus