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Quantitative analysis of nanoparticle transport through in vitro blood-brain barrier models

View Article: PubMed Central - PubMed

ABSTRACT

Nanoparticle transport through the blood-brain barrier has received much attention of late, both from the point of view of nano-enabled drug delivery, as well as due to concerns about unintended exposure of nanomaterials to humans and other organisms. In vitro models play a lead role in efforts to understand the extent of transport through the blood-brain barrier, but unique features of the nanoscale challenge their direct adaptation. Here we highlight some of the differences compared to molecular species when utilizing in vitro blood-brain barrier models for nanoparticle studies. Issues that may arise with transwell systems are discussed, together with some potential alternative methodologies. We also briefly review the biomolecular corona concept and its importance for how nanoparticles interact with the blood-brain barrier. We end with considering future directions, including indirect effects and application of shear and fluidics-technologies.

No MeSH data available.


Potential issues with applying transwell systems to measure the transport of nanoparticles across in vitro blood-brain barriers.
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f0002: Potential issues with applying transwell systems to measure the transport of nanoparticles across in vitro blood-brain barriers.

Mentions: This methodology has a long history and, while there may of course be technical complications in specific cases, is well-established as a general tool for measuring transport of molecular species. However, several issues arise when applying the same methodology to the transport of nanoparticles across barriers (Fig. 2). Some of these issues are not novel for nanoscale objects (e.g., imperfections of the barriers) but would appear to be more severe for assessing transport of nanoparticles; some of the issues (e.g., agglomeration) are, on the other hand, rather unique for particles.Figure 2.


Quantitative analysis of nanoparticle transport through in vitro blood-brain barrier models
Potential issues with applying transwell systems to measure the transport of nanoparticles across in vitro blood-brain barriers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836482&req=5

f0002: Potential issues with applying transwell systems to measure the transport of nanoparticles across in vitro blood-brain barriers.
Mentions: This methodology has a long history and, while there may of course be technical complications in specific cases, is well-established as a general tool for measuring transport of molecular species. However, several issues arise when applying the same methodology to the transport of nanoparticles across barriers (Fig. 2). Some of these issues are not novel for nanoscale objects (e.g., imperfections of the barriers) but would appear to be more severe for assessing transport of nanoparticles; some of the issues (e.g., agglomeration) are, on the other hand, rather unique for particles.Figure 2.

View Article: PubMed Central - PubMed

ABSTRACT

Nanoparticle transport through the blood-brain barrier has received much attention of late, both from the point of view of nano-enabled drug delivery, as well as due to concerns about unintended exposure of nanomaterials to humans and other organisms. In vitro models play a lead role in efforts to understand the extent of transport through the blood-brain barrier, but unique features of the nanoscale challenge their direct adaptation. Here we highlight some of the differences compared to molecular species when utilizing in vitro blood-brain barrier models for nanoparticle studies. Issues that may arise with transwell systems are discussed, together with some potential alternative methodologies. We also briefly review the biomolecular corona concept and its importance for how nanoparticles interact with the blood-brain barrier. We end with considering future directions, including indirect effects and application of shear and fluidics-technologies.

No MeSH data available.