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Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic effects of chaetocin on cell viability of generated CIK cells. The cells were incubated in medium containing 0–10 nM chaetocin and the cell viability was determined after 1 day (red), after 3 days (green) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
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Figure 4: Cytotoxic effects of chaetocin on cell viability of generated CIK cells. The cells were incubated in medium containing 0–10 nM chaetocin and the cell viability was determined after 1 day (red), after 3 days (green) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.

Mentions: In order to use chaetocin in combination with CIK cells it must be ensured that the used chaetocin concentrations have minimal or no effects on the viability of the CIK cells. Figure 4 (Fig. 4) displays the effects of chaetocin on CIK cells when incubated up to four days with different concentrations. The cell viability decreased to 91% after one day incubation with 10 nM chaetocin. The decrease progresses slowly until a cell viability of 75% is reached at 100 nM and 200 nM. The final concentration of 400 nM still leaves 67% of the cells viable. After three and four days of incubation at a concentration of 10 nM chaetocin 71 % of the cells were still alive in both samples. The cell viability of both samples decreased slowly and steady to 56% at 100 nM. The incubation with 200 nM and 400 nM show the first difference between the three and four days of incubation because the cells that were incubated for three days only show a viability of 22% while the four days of incubation resulted in a viability of 53%.


Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Cytotoxic effects of chaetocin on cell viability of generated CIK cells. The cells were incubated in medium containing 0–10 nM chaetocin and the cell viability was determined after 1 day (red), after 3 days (green) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836455&req=5

Figure 4: Cytotoxic effects of chaetocin on cell viability of generated CIK cells. The cells were incubated in medium containing 0–10 nM chaetocin and the cell viability was determined after 1 day (red), after 3 days (green) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
Mentions: In order to use chaetocin in combination with CIK cells it must be ensured that the used chaetocin concentrations have minimal or no effects on the viability of the CIK cells. Figure 4 (Fig. 4) displays the effects of chaetocin on CIK cells when incubated up to four days with different concentrations. The cell viability decreased to 91% after one day incubation with 10 nM chaetocin. The decrease progresses slowly until a cell viability of 75% is reached at 100 nM and 200 nM. The final concentration of 400 nM still leaves 67% of the cells viable. After three and four days of incubation at a concentration of 10 nM chaetocin 71 % of the cells were still alive in both samples. The cell viability of both samples decreased slowly and steady to 56% at 100 nM. The incubation with 200 nM and 400 nM show the first difference between the three and four days of incubation because the cells that were incubated for three days only show a viability of 22% while the four days of incubation resulted in a viability of 53%.

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus