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Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic effects of chaetocin on cell viability of non-cancerous cell line CCD-18Co. The cells were incubated in medium containing 0–400 nM chaetocin and the cell viability was determined after 1 day (red) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
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Figure 3: Cytotoxic effects of chaetocin on cell viability of non-cancerous cell line CCD-18Co. The cells were incubated in medium containing 0–400 nM chaetocin and the cell viability was determined after 1 day (red) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.

Mentions: CCD-18Co is a non-cancerous cell line originated from the human colon. This cell line is used to test the cytotoxic effects of chaetocin on non-cancerous cells in order to compare it with the effects on ccRCC cell lines. Figure 3 (Fig. 3) shows the results of the MTT test for non-cancerous colon cells that were incubated with different concentrations of chaetocin. The cell viability stays above 80% until a concentration of 200 nM is reached. Incubation with 200 nM chaetocin results in the death of 28% while a concentration of 400 nM kills 91% of the cells when incubated for 24h. Four days of incubation resulted in a drop of cell viability to 73% after administration of 10 nM chaetocin. The next drop in cell viability is observed at 100 nM where 66% of the cells were still alive while it was only 50% for 200 nM of chaetocin. The final concentration of 400 nM resulted in 21% cell viability which is more than twice the viability of the samples that were incubated for one day.


Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Cytotoxic effects of chaetocin on cell viability of non-cancerous cell line CCD-18Co. The cells were incubated in medium containing 0–400 nM chaetocin and the cell viability was determined after 1 day (red) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836455&req=5

Figure 3: Cytotoxic effects of chaetocin on cell viability of non-cancerous cell line CCD-18Co. The cells were incubated in medium containing 0–400 nM chaetocin and the cell viability was determined after 1 day (red) and after 4 days (blue) by the use of the MTT assay. Triplicates were used for each sample to determine the mean ±SD. The significance was determined by the use of t-test (*=0.05–0.04, **=0.02–0.03, ***=<0.002). Cells without chaetocin treatment were used as negative control while cells treated with a deadly dose of DMSO were used as positive control.
Mentions: CCD-18Co is a non-cancerous cell line originated from the human colon. This cell line is used to test the cytotoxic effects of chaetocin on non-cancerous cells in order to compare it with the effects on ccRCC cell lines. Figure 3 (Fig. 3) shows the results of the MTT test for non-cancerous colon cells that were incubated with different concentrations of chaetocin. The cell viability stays above 80% until a concentration of 200 nM is reached. Incubation with 200 nM chaetocin results in the death of 28% while a concentration of 400 nM kills 91% of the cells when incubated for 24h. Four days of incubation resulted in a drop of cell viability to 73% after administration of 10 nM chaetocin. The next drop in cell viability is observed at 100 nM where 66% of the cells were still alive while it was only 50% for 200 nM of chaetocin. The final concentration of 400 nM resulted in 21% cell viability which is more than twice the viability of the samples that were incubated for one day.

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus