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Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus

Viability of tumor cell lines CAKI-2 (blue, left), A-498 (red, middle) and non-cancerous cell line CCD-18Co (green, right) after 24 h of exposure to chaetocin concentration in the range of 0–10 nM determined with a micro plate reader after MTT assay. Positive control; cell lines killed with DMSO. Negative control; cells without any treatment.
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Figure 1: Viability of tumor cell lines CAKI-2 (blue, left), A-498 (red, middle) and non-cancerous cell line CCD-18Co (green, right) after 24 h of exposure to chaetocin concentration in the range of 0–10 nM determined with a micro plate reader after MTT assay. Positive control; cell lines killed with DMSO. Negative control; cells without any treatment.

Mentions: Prior research stated that 24 h exposure of 2–10 nM chaetocin cause the death of 50% of all tested solid tumor cell lines [11]. So the first step was a chaetocin tittering in the range of 0–10 nM with two tumor (A-498, CAKI-2) and one non-cancerous control cell line (CCD-18co) (see Figure 1 (Fig. 1)). The results of the MTT assay clearly show that the cell viability of CCD-18Co non-cancerous cell line is not affected by the chosen chaetocin concentration. The ccRCC cell line CAKI-2 shows a small drop in cell viability to 92% at a concentration of 10 nM chaetocin while the A-498 tumor cell lines viability drops to 86% at a chaetocin concentration of 5 nM and even to 77% at a concentration of 10 nM chaetocin.


Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells.

Rombo R, Weiher H, Schmidt-Wolf IG - Ger Med Sci (2016)

Viability of tumor cell lines CAKI-2 (blue, left), A-498 (red, middle) and non-cancerous cell line CCD-18Co (green, right) after 24 h of exposure to chaetocin concentration in the range of 0–10 nM determined with a micro plate reader after MTT assay. Positive control; cell lines killed with DMSO. Negative control; cells without any treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836455&req=5

Figure 1: Viability of tumor cell lines CAKI-2 (blue, left), A-498 (red, middle) and non-cancerous cell line CCD-18Co (green, right) after 24 h of exposure to chaetocin concentration in the range of 0–10 nM determined with a micro plate reader after MTT assay. Positive control; cell lines killed with DMSO. Negative control; cells without any treatment.
Mentions: Prior research stated that 24 h exposure of 2–10 nM chaetocin cause the death of 50% of all tested solid tumor cell lines [11]. So the first step was a chaetocin tittering in the range of 0–10 nM with two tumor (A-498, CAKI-2) and one non-cancerous control cell line (CCD-18co) (see Figure 1 (Fig. 1)). The results of the MTT assay clearly show that the cell viability of CCD-18Co non-cancerous cell line is not affected by the chosen chaetocin concentration. The ccRCC cell line CAKI-2 shows a small drop in cell viability to 92% at a concentration of 10 nM chaetocin while the A-498 tumor cell lines viability drops to 86% at a chaetocin concentration of 5 nM and even to 77% at a concentration of 10 nM chaetocin.

Bottom Line: The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells.Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin.Abstract available from the publisher.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Integrated Oncology (CIO), University of Bonn, Bonn, Germany; Hochschule Bonn-Rhein-Sieg, Rheinbach, Germany.

ABSTRACT
We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.

No MeSH data available.


Related in: MedlinePlus